FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16942
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT4282.526266084287569No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1330.7850312832015112No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT1310.7732263014992327RNA PCR Primer, Index 27 (96% over 25bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA1260.7437138472435368No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1120.6610789753275882RNA PCR Primer, Index 27 (95% over 23bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG720.42497934128202103No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG550.3246369968126549No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG530.31283201511037656No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG510.3010270334080982No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG490.2892220517058198RNA PCR Primer, Index 27 (100% over 24bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA480.2833195608546807No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG470.2774170700035415No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT460.2715145791524023No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC380.22429465234328885No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC370.21839216149214966No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC310.1829772163853146No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC220.12985479872506198No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT210.1239523078739228No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG200.11804981702278361No Hit
CCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT200.11804981702278361No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.11214732617164443No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC190.11214732617164443No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC190.11214732617164443No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT180.10624483532050526No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC180.10624483532050526No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT180.10624483532050526No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTGT170.10034234446936609RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)