FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11516
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA1231.0680791941646406No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT1070.9291420632163946No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGA760.6599513720041681No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGA650.5644320944772491No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACAT580.5036470996873914No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACAT560.48627995831886067No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG550.47759638763459533No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCT540.46891281695033No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCG530.4602292462660646No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTAT510.44286210489753386No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG510.44286210489753386No Hit
TCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCG480.4168113928447377No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA460.399444251476207No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTT460.399444251476207No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTA450.39076068079194165No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG450.39076068079194165No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATA440.38207711010767625No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACAT430.3733935394234109No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACAT430.3733935394234109No Hit
CATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC430.3733935394234109No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCG410.3560263980548802No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTC390.33865925668634944No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC380.32997568600208405No Hit
GCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACG370.3212921153178187No Hit
AGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCG370.3212921153178187No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTT370.3212921153178187No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATA370.3212921153178187No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG360.3126085446335533No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTA350.30392497394928797No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGA330.2865578325807572No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGT330.2865578325807572No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGA330.2865578325807572No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTAT330.2865578325807572No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCG320.27787426189649184No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC300.2605071205279611No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTT300.2605071205279611No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTC300.2605071205279611No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTG290.2518235498436957No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATC290.2518235498436957No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCG290.2518235498436957No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCG290.2518235498436957No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTC290.2518235498436957No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCT290.2518235498436957No Hit
CTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTG290.2518235498436957No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTC290.2518235498436957No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTC280.24313997915943034No Hit
AGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC280.24313997915943034No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCT280.24313997915943034No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCG270.234456408475165No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACAT260.22577283779089963No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCG260.22577283779089963No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTG260.22577283779089963No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC260.22577283779089963No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGT250.21708926710663426No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATC250.21708926710663426No Hit
CCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATT250.21708926710663426No Hit
GTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGT240.20840569642236886No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACAT240.20840569642236886No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACA230.1997221257381035No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC230.1997221257381035No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACA230.1997221257381035No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGA230.1997221257381035No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATA220.19103855505383813No Hit
GTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTC220.19103855505383813No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTC210.18235498436957276No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG210.18235498436957276No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATA210.18235498436957276No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC200.1736714136853074No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC200.1736714136853074No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATA200.1736714136853074No Hit
GCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATC200.1736714136853074No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCT200.1736714136853074No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTT200.1736714136853074No Hit
GAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGAC200.1736714136853074No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCT200.1736714136853074No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACAT190.16498784300104202No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCT190.16498784300104202No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACAT190.16498784300104202No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT190.16498784300104202TruSeq Adapter, Index 1 (95% over 22bp)
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGT190.16498784300104202No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGA190.16498784300104202No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC190.16498784300104202No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT190.16498784300104202RNA PCR Primer, Index 1 (95% over 24bp)
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTC180.15630427231677665No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC180.15630427231677665No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT180.15630427231677665No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCT180.15630427231677665No Hit
TCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCA180.15630427231677665No Hit
GTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCA180.15630427231677665No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGT170.1476207016325113No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC170.1476207016325113No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACA170.1476207016325113No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTT170.1476207016325113No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCT170.1476207016325113No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC170.1476207016325113No Hit
CTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACG170.1476207016325113No Hit
CCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACC170.1476207016325113No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACA170.1476207016325113No Hit
GATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGAT170.1476207016325113No Hit
ATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGC170.1476207016325113No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATA160.13893713094824592No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTC160.13893713094824592No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTAT160.13893713094824592No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGT160.13893713094824592No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTC160.13893713094824592No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCT160.13893713094824592No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT160.13893713094824592No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTT160.13893713094824592No Hit
GCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACC150.13025356026398055No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTC150.13025356026398055No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT150.13025356026398055No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCT150.13025356026398055No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC150.13025356026398055No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCT150.13025356026398055No Hit
CTTATACACATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC150.13025356026398055No Hit
GGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGA150.13025356026398055No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCC150.13025356026398055No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGA150.13025356026398055No Hit
AGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGA150.13025356026398055No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTC150.13025356026398055No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGCTGTCTCTTATACACATCTC150.13025356026398055No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACA150.13025356026398055No Hit
TCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCC150.13025356026398055No Hit
TTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATC150.13025356026398055No Hit
AGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCG140.12156998957971517No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC140.12156998957971517No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTC140.12156998957971517No Hit
CATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTC140.12156998957971517No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTC140.12156998957971517No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACAT140.12156998957971517No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG140.12156998957971517No Hit
TTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATAC140.12156998957971517No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT140.12156998957971517No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA140.12156998957971517No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACAC140.12156998957971517No Hit
GGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCC140.12156998957971517No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.12156998957971517No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG140.12156998957971517No Hit
CGAGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTC130.11288641889544981No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC130.11288641889544981No Hit
GTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCC130.11288641889544981No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATC130.11288641889544981No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGC130.11288641889544981No Hit
GTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGA130.11288641889544981No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACA130.11288641889544981No Hit
GCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCC130.11288641889544981No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT130.11288641889544981No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCT130.11288641889544981No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTC130.11288641889544981No Hit
GAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAG130.11288641889544981No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACA120.10420284821118443No Hit
ACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGC120.10420284821118443No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTG120.10420284821118443No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTCTGTCTCTTATACACATCTCC120.10420284821118443No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT120.10420284821118443No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGA120.10420284821118443No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGT120.10420284821118443No Hit
TGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCC120.10420284821118443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)