FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172882
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT68223.94604412258072No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA57913.349683599217964No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG54993.1807822676739046No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG48122.7834013951712726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC32301.868326372901748No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG28931.6733957265649402No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG21311.2326326627410602No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA17791.029025578140003No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT16560.9578787843731562RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT15700.9081338716581252RNA PCR Primer, Index 17 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA14950.8647516803368772No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT13660.7901343112643306No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA11400.6594093080829698No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC11110.6426348607720873No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT10230.591733089621823No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT10150.5871056558808899No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9300.5379391723834754No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT9270.5362038847306255No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT8760.5067039946321769No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT8540.49397855184461076No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA8380.48472368436274454No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG8340.482409967492278No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG8240.4766256753161116No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC7970.46100808644046226No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC7460.43150819634201365No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC7350.42514547494823063No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT7320.4234101872953807No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC7300.4222533288601474No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG6960.4025867354611816TruSeq Adapter, Index 12 (95% over 23bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT6900.3991161601554818No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT6790.3927534387616987No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAG6630.38349857127983245No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC6370.36845941162179985No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA6280.3632535486632501No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG6030.3487928182228341No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA6010.3476359597876008No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT5980.3459006721347509No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCA5730.3314399416943349No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC5700.3297046540414849No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG5660.32739093717101836No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT5430.3140870651658357No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT5380.31119491907775243No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5350.30945963142490257No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT5150.2978910470725697No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG5110.29557733020210314No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC4890.2828518874145371No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA4750.2747538783679041No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA4660.26954801540935436No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT4640.2683911569741211No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA4380.25335199731608843No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT4350.2516167096632385No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT4290.24814613435753868No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4150.2400481253109057No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC4050.23426383313473928No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG3920.22674425330572295No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA3910.22616582408810634No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA3880.2244305364352564No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCC3750.21691095660624007No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT3650.2111266644300737No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC3520.20360708460105736No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC3440.19897965086012426No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT3430.19840122164250762No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCA3420.19782279242489095No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA3380.1955090755544244No Hit
GGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT3310.19146007103110793No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG3310.19146007103110793No Hit
CAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC3230.1868326372901748No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTTCT3180.1839404912020916RNA PCR Primer, Index 19 (95% over 21bp)
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3150.18220520354924166No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC3110.1798914866787751No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTTTGCCGT3100.1793130574611585No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG3100.1793130574611585No Hit
GTCCAGGGTGGGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAG3090.17873462824354183No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA3040.1758424821554586No Hit
CATCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA3000.17352876528499206No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC2980.17237190684975878No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT2950.17063661919690887No Hit
GCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG2920.16890133154405895No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT2920.16890133154405895No Hit
GCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA2890.16716604389120904No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA2880.1665876146735924No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2810.1625386101502759No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC2800.16196018093265926No Hit
GGTCACGTCCTTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG2790.16138175171504263No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2660.1538621718860263No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG2650.15328374266840966No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC2650.15328374266840966No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA2590.14981316736270983No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA2540.1469210212746266No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG2530.14634259205701No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG2450.14171515831607687No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCT2440.14113672909846023No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA2380.1376661537927604No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT2350.13593086613991046No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT2320.13419557848706054No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATTCC2280.13188186161659396No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA2280.13188186161659396No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC2270.13130343239897735No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTT2200.12725442787566085No Hit
CCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2190.12667599865804421No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT2190.12667599865804421No Hit
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA2170.12551914022281094No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2170.12551914022281094RNA PCR Primer, Index 17 (96% over 26bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA2140.12378385256996101No Hit
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA2110.12204856491711108No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA2100.12147013569949446No Hit
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA2090.1208917064818778No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC2090.1208917064818778No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC2090.1208917064818778No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA2090.1208917064818778No Hit
CTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA2080.12031327726426118No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTTTGCCGT2060.1191564188290279No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2040.11799956039379461No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGA1970.11395055587047813No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG1960.11337212665286148No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG1890.10932312212954501No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT1880.10874469291192836No Hit
CATCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC1870.10816626369431173No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC1860.10758783447669508No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT1850.10700940525907846No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGGCGT1840.1064309760414618No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT1830.10585254682384518No Hit
TTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA1820.10527411760622853No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATTCCGT1810.1046956883886119No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT1800.10411725917099525No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT1800.10411725917099525No Hit
CCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT1760.10180354230052868No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC1750.10122511308291203No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1740.1006466838652954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)