FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439452
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA179984.095555373510646No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG175403.99133466226118No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG163923.730100215723219No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG89412.034579430745565No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA60801.383541319643556No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT48281.0986410347432711No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC46081.048578684361432No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG45371.032422198556384No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT41820.9516397695311433No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT39440.8974814086635172No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT35680.8119203007381922No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT30040.6835786388502044No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC29930.6810755213311124No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC29760.6772070669834248No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT29600.6735661687738365No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT29330.6674221530451562No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT28530.6492176619972148No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC28320.64443898309713No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC27880.6344265130207623No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG27670.6296478341206776No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT27100.6166771342490193No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC26770.6091677816917433No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC22870.5204208878330284No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC22650.5154146527948444No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC22420.5101808616185614No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC19330.4398660149458872No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT18920.4305362132838171No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC18300.41642773272166245No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC18190.41392461520257046No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA18150.4130143906501734No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC17910.40755304333579095No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA17360.39503745574033117No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT16390.372964510344702No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG15660.3563529122634554No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC15450.35157423336337074No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT15310.348388447429981No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT14350.32654305817245116No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT13910.3165305880960833No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC13630.31015901622930375No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTGGCGG13410.3051527811911198No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA12990.2955954233909505No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12780.2908167444908659No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC12370.2814869428287959No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG12210.27784604461920753No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA11980.27261225344292433No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT11960.27215714116672585No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC11940.2717020288905273No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA11530.26237222722845727No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC11120.25304242556638723No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA11030.2509944203234938No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10960.2494015273567989No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT10830.24644329756150843No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC10830.24644329756150843No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC10820.24621574142340916No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT10810.2459881852853099No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT10270.23370015382794934No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA9940.22619080127067348No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGCGG9860.2243703521658793No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC9780.22254990306108516No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG9730.22141212237058883No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA9640.2193641171276954No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA9460.21526810664190854No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG9310.21185476457041952No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9140.20798631022273195No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT9130.20775875408463265No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT8850.20138718221785315No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGGTGGGGG8790.20002184538925752No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT8780.19979428925115827No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC8710.19820139628446337No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT8570.19501561035107362No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC8340.18978181917479042No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT8290.1886440384842941No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGC8240.18750625779379773No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA8100.18432047186040795No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC8090.1840929157223087No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC8070.18363780344611016No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT7930.1804520175127204No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT7720.17567333861263573No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAT7720.17567333861263573No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT7510.1708946597125511No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA7340.1670262053648635No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT7130.16224752646477886No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG6800.15473817390750297No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC6610.15041460728361689No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG6570.1495043827312198No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC6520.14836660204072344No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT6390.14540837224543296No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG6340.1442705915549366No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA6200.14108480562154685No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG6080.13835413196435561No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGGTGGCGG6060.13789901968815707No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG6050.1376714635500578No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG6030.13721635127385928No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT5880.13380300920237023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTTTTGC5770.13129989168327827No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT5750.13084477940707973No Hit
GCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC5730.13038966713088118No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC5680.12925188644038485No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA5650.12856921802608703No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC5570.1267487689212929No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT5540.12606610050699507No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT5540.12606610050699507No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA5520.12561098823079656No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT5520.12561098823079656No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGGTTGC5440.12379053912600238No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA5430.12356298298790312No Hit
GGTCACGTCCTTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC5390.12265275843550605No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG5320.12105986546881115No Hit
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA5280.12014964091641407No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT5260.11969452864021556No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA5250.11946697250211627No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA5220.11878430408781845No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG5200.11832919181161994No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTTTGGCGG5020.11423318132583309No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC4990.11355051291153527No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC4960.11286784449723747No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC4950.1126402883591382No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC4930.11218517608293965No Hit
GTCCAGGGTGGGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC4910.11173006380674114No Hit
CATCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA4870.11081983925434404No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT4850.1103647269781455No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA4800.10922694628764916No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT4740.10786160945905354No Hit
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA4700.10695138490665647No Hit
GCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA4640.10558604807806085No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT4580.10422071124946523No Hit
CCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT4540.10331048669706816No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGA4500.10240026214467109No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4490.10217270600657183No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC4480.10194514986847254No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC4460.10149003759227403No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTTTTGCGG4440.10103492531607547No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGGTTGG4400.10012470076367841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)