FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT27.6923076923076925No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG27.6923076923076925No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG27.6923076923076925No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA27.6923076923076925No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGAGGATGGGTAGGGGGAT13.8461538461538463No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA13.8461538461538463No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT13.8461538461538463No Hit
CTTTTACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAATTAGG13.8461538461538463No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATGTCCGAGCGCACCAGACTAGCGCTCATCTCGTATGCCGTCTTGT13.8461538461538463Illumina PCR Primer Index 7 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAATCAGCACTCTGCACAGCATCATCCAT13.8461538461538463No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA13.8461538461538463No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCCTCTCCACGTTGCGCGCTTGGCCTTGTCGCGGCTCTTG13.8461538461538463No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAAATTTTGATGGTCGACATTATTATTTTATTGNCATGTGCCAATA13.8461538461538463No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCGTCCAT13.8461538461538463No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCTCGAATGGGCGCTG13.8461538461538463No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCACCTCCCGGGTGCGGGGGGCACCCTCAGGGA13.8461538461538463No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCAAATTTCAGCTCCATTGGGATCATG13.8461538461538463No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTGTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACC13.8461538461538463No Hit
ATAGTACTGCTCGTACAGGTAGCAGGCAACAACAATACTGGAGGAGACCGTGTAGAGAAGCGTGAGGATACCGAA13.8461538461538463No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACTGCCCC13.8461538461538463No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACATTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG13.8461538461538463No Hit
GTTTCCAGGTCACGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGTTGCGGGGGGCCCCCTCAGTGA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)