FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT1219.047619047619047RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT69.523809523809524RNA PCR Primer, Index 45 (96% over 25bp)
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT34.761904761904762RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT34.761904761904762RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTCATTCATCTCGTATGCCGTCTT23.1746031746031744RNA PCR Primer, Index 45 (100% over 23bp)
ATAGCAGAATCATCGTGGTCTCCTACAACTTCTGAAGGCTGTCTCTTATACCCATCTCCGAGCCCACGAGACGTG11.5873015873015872No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATTCCGTCTT11.5873015873015872No Hit
CAAGCACTCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT11.5873015873015872RNA PCR Primer, Index 45 (96% over 25bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAACTCGNAGGCCGTCTTCT11.5873015873015872No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGCCGTTCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (100% over 23bp)
CAACCAGAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTNGTATGCCGTCTT11.5873015873015872No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCATCTT11.5873015873015872No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCTAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACCCATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATTCCGTCTT11.5873015873015872No Hit
CAACCAAAAGAACAGCCTTTCTCTTAGACGCAGCTCCGAGCCCACGAGACGTACATTCATCGCGGATGACGTCTT11.5873015873015872No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGNATGCAGTCTTCT11.5873015873015872No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCTTCTT11.5873015873015872No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCACACAAGACGTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (100% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACGTGCATTCATATCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCTTCTT11.5873015873015872No Hit
CAACCACAAGAACAGCCTGTCTCTTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCT11.5873015873015872RNA PCR Primer, Index 45 (100% over 21bp)
CAACCAAAAGAACAGCCTGTCGCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (100% over 22bp)
AAACTCCCTGACCAATTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTC11.5873015873015872RNA PCR Primer, Index 45 (95% over 24bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTCATTCATCTCGTATGCCGGCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACGTGCATTCATCTCGCATGCCGTCTA11.5873015873015872No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGNATGCCGTCTTCT11.5873015873015872TruSeq Adapter, Index 7 (95% over 21bp)
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCGTCTCGTATGCCGTCGTCT11.5873015873015872No Hit
CAAGCAATAAAGGCCAGTCTCTATACACATCGACGAGCCCACGAGACGGGCATACAAATCGTATGCCGGATACAG11.5873015873015872No Hit
CAAGCAAAAGAACCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCCTCTCGTATGCCGTCTT11.5873015873015872No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAGCTCGTATGCCGGCTT11.5873015873015872No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAGCTCGTATGCCGTCTT11.5873015873015872No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGACTT11.5873015873015872No Hit
CAACCAAACTAACAGCCTGTCTCTTATACCCATCTCCTAGCCCACGAGACGTTCATTCATCTCGTATACCATCTT11.5873015873015872No Hit
CAACCAAAATAGCAGCCTGTCTCTTATACACATCTCCAAGCCCACTAGACTTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (100% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAACTCGTATGCCGTCTTCT11.5873015873015872TruSeq Adapter, Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTTTCTCTTATACACATCTCCGAGCCCACGAGACGTTCATTCATCTNGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 23bp)
CCCTCCCCCTCCCCTCCTTTCTCTTCTACACCTCTCCAGCCCACTAGACGTGCATTCATCTCGTATGCCGTCTTC11.5873015873015872RNA PCR Primer, Index 45 (100% over 23bp)
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.5873015873015872RNA PCR Primer, Index 45 (95% over 23bp)
GCACAGGTCGTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCNGCTTGAAAAAAA11.5873015873015872TruSeq Adapter, Index 7 (96% over 26bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAAACGTGCATTCAGCTCGTATGCCTTCTT11.5873015873015872No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGACGTCTG11.5873015873015872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)