FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT714.893617021276595No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCT36.382978723404255Illumina PCR Primer Index 10 (95% over 22bp)
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC24.25531914893617No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAGCTCGTATGCCGTCTT24.25531914893617No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGAACTCATCTCAACTCGTATGCCGTCTTCT12.127659574468085Illumina PCR Primer Index 7 (95% over 21bp)
CAACCAAAATAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCATCCCATCTCGTATGCCGTCTT12.127659574468085RNA PCR Primer, Index 14 (95% over 21bp)
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCACGCAACATCCTCTCAACTCGTATTC12.127659574468085No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAGCTCGTATGCCGTCTT12.127659574468085No Hit
ATAGCAGAATCATCGTGGTCTCCTACAACTTCTGAAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGGCATCATCTCATCTCGTATGCCGTCTT12.127659574468085No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGACATCATCTCATCTCGTATGCCGTCTT12.127659574468085No Hit
CAACCAAAAGAACCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATATCGTATGCAGTCTT12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTAATATCGTATGCCGTCTT12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAACTCGTATGCCGTCTT12.127659574468085No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA12.127659574468085Illumina PCR Primer Index 10 (96% over 27bp)
CAACAAAAAGAACAGCCTGTCTCTTATACACATGTCCGAGCCCACGAGACATCATCGCAGGTCGTATGCCGGCTT12.127659574468085No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC12.127659574468085No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT12.127659574468085No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCCTATT12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATATCGTATGCCGTCTT12.127659574468085No Hit
CAACCAAAAGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCACATCTCGTATGCCGTCTTC12.127659574468085TruSeq Adapter, Index 9 (95% over 23bp)
CAACCCAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTCTGCCGTCTT12.127659574468085No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCTTCTT12.127659574468085No Hit
CAAGCAACCGCCCCGCCTTTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCCTCTCGTATGCCGTCTT12.127659574468085No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA12.127659574468085No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG12.127659574468085No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGATGCGGGGGGCCCCCTCAGTGA12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTGTACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT12.127659574468085No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG12.127659574468085No Hit
CAACCAAAAGACCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCACCTCGTATGCCGTCTT12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCACCTCGTATGCCGTCTT12.127659574468085No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCGCACGAGACATCGTCTCATCTCGTATGCCGTCGT12.127659574468085No Hit
CTTCTTGTTTTCGTGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC12.127659574468085No Hit
CAACCAAAAGAATAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACATAATCTCATCTCGTATGCCGTCTT12.127659574468085No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATATCGTATGCCGTCTTCT12.127659574468085Illumina PCR Primer Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATCACATCTCCGAGCCCACGAGACATCATCTCAGCTCGTATGCCGTCTTC12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)