FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG1313.684210526315791No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG99.473684210526317No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG22.1052631578947367No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC22.1052631578947367No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT22.1052631578947367No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT22.1052631578947367No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT22.1052631578947367No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT22.1052631578947367No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT22.1052631578947367No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG22.1052631578947367No Hit
CAAGCAATCAAGGCCAGGCTACCCTGCAGCCCTTGCCGCCTTCCCATACGCAGCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCAGCCAG11.0526315789473684No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGGTAAAAAACGGAGTGGTGCCATTCAGGTA11.0526315789473684No Hit
TAAAATAGTAGCCCTCCAGCACTCACCAGGTCCGGTCGGTAGTACCTGTTCACCACTTCTGCCCCTGCGCACATG11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGGCACAGTTTCTCCCCAATGCGACGAACTTCTTTTTTGTTATCAGCCCAGT11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGCTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG11.0526315789473684No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTAAGCAAACACTCGGAAGAAGTAGG11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCTGTAGTACATGTGCACCACTTCTGCCCCTTCTCCCATT11.0526315789473684No Hit
GCACAGGTCGTATACGCAGTGGCGGAAGGACTCAGAGGGACTCAGCACCGCGTGGCAGGTCGCGAAGGGGCCCTG11.0526315789473684No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTATCTTCTTTTTTCTTCCTGGCAGTATTAT11.0526315789473684No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGACAGTATTAT11.0526315789473684No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGGGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA11.0526315789473684No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGCGGCTGCGCGGGGCCCGCGCCCC11.0526315789473684No Hit
CAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCAAAGCATCATCCATCCAT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAACAGCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCACACTCACAAGGTCCGATCGATAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG11.0526315789473684No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT11.0526315789473684No Hit
AACTTTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT11.0526315789473684No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA11.0526315789473684No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGACGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA11.0526315789473684No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGGCTGGGTTGTTATCAGCCAAGT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCACTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCCCCACTTCTGCCCCTGCGCACATG11.0526315789473684No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT11.0526315789473684No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGATCGTGTAGAGCAGCGCGAAGATGCTGAT11.0526315789473684No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCACGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGGGA11.0526315789473684No Hit
AACTTTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGATACGAACGTCTGTGTTTTTATCAGCCAAGC11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGTAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCCTAAACAGCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCNGGTAGCCGGCAA11.0526315789473684No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATACGCATCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGGCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATGATCCAT11.0526315789473684No Hit
CTTTTACAGCATCACGAGTTTCACCTGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCGCTTGCCACCTTCCGATAAGCAGCACTCTGCACAGCATCATCCAT11.0526315789473684No Hit
AACTGTAGCTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGCT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACCGCATCATCCAT11.0526315789473684No Hit
CTTTGACAGCATACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGGA11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTACGGTCGGTAGTACCTGTGCACCACTTCTGCCCATGCGCACATG11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCATCACTCTGCANAACATCATCCAT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAACAGCACTCTGCACAGCATAATCAAT11.0526315789473684No Hit
CAGTGACATGTATTCCCCCTCTTACCTTACGGCTGTGCTTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.0526315789473684No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACAACTTCTGCCCCTGCGCACATG11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC11.0526315789473684No Hit
ATAGCAAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCATGGGAAGCAGTGGC11.0526315789473684No Hit
AACTGTAGCTTACACTGCCTCCGTGGAACCGTTTCGCCCCAATGAGACGAACGGCTGTGTTGNTATCAGCCGAGT11.0526315789473684No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC11.0526315789473684No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCACTATGCTGTAGTACCGCATTTCCTACCTGA11.0526315789473684No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACATAGCCAACGTCCTGGAAGGCGGGGACCCCACCTTCCCAC11.0526315789473684No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGAGCCCTG11.0526315789473684No Hit
GTAGTGCTGCTAGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT11.0526315789473684No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACACTTTCAACAACTCCTGTT11.0526315789473684No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGACAAGT11.0526315789473684No Hit
AACTGTAGCTTACACTGACTCCGTGGCACAGTTTCTCCCCGATGAGACCAACGTCTGTGTTGTTATCAGCCAAGC11.0526315789473684No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)