FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10475
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG571754.57756563245824No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG106910.205250596658711No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG4564.353221957040573No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC3453.2935560859188544No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC3443.2840095465393793No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2502.386634844868735No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC1921.8329355608591884No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC1701.6229116945107398No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT1361.2983293556085918No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT1291.2315035799522671No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG1171.1169451073985681No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC710.6778042959427207No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA620.5918854415274463No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT470.4486873508353222No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC420.40095465393794755No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG360.3436754176610979No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG340.32458233890214794No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT280.26730310262529833No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC210.20047732696897377No Hit
ACACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG200.1909307875894988No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.1909307875894988No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.16229116945107397No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCA160.15274463007159905No Hit
GCACAGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGC140.13365155131264916No Hit
GCACAGGCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGC140.13365155131264916No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGTACCG130.12410501193317422No Hit
GCACAGGTCATATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG130.12410501193317422No Hit
GCACAGATCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG130.12410501193317422No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACCCAGCACCG130.12410501193317422No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG120.11455847255369928No Hit
GCACAGGTCGTATACGCCTTGGCGGAAGTACTCAGAGGGACTCAGCACCG120.11455847255369928No Hit
GCACAGGTCGTATACGCATTGGCGGCAGTACTCAGAGGGACTCAGCACCG110.10501193317422435No Hit
GCACAGGCCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG110.10501193317422435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)