FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53212
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG41297.759527926031722No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG35436.658272570096971No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC33696.331278658949109No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT32386.085093587912501No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC31135.850184168984439No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT27835.230023303014358No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA26494.978200405923476No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT26344.950011275652109No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG26074.899270841163647No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG23044.3298504096820265No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT20343.8224460647974143No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG18313.4409531684582424No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG15872.9824099827106667No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC14642.7512591144854546No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG13862.604675637074344No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG13052.4524543336089604No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC12682.3829211456062542No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG10782.025858828835601No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC10591.9901525971585357No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT10001.8792753514244906No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA9321.7514846275276255No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT8481.593625498007968No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA4730.8888972412237841No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT3700.6953318800270616No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT3570.6709013004585431No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT1460.27437420130797563No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCTTG840.15785912951965722No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG740.1390663760054123No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT650.1221528978425919No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT600.11275652108546945No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCTGCT600.11275652108546945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)