FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002295673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799797
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG181670.6488684715356149No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA150080.5360388628175543No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG104090.3717769538291526No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG78970.28205616335755773No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT78190.2792702470929142No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG73230.26155467699979673No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA64930.23190967059397521No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA52040.18587061847698244No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC51130.18262038283489837No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT48170.1720481877793283No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT42760.15272535830276268No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT42300.15108238204412677No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC41380.14779642952685498No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAAA39210.1400458676111161No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC38140.13622416196602824No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC32310.11540122373157768No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31970.11418684997519463Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31610.1129010424684361Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT30340.10836499931959354No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30280.10815069806846711Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC29040.10372180554518774No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC28770.10275744991511886No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT28740.10265029928955564No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT28640.10229313053767826No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC28470.10168594365948674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)