FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564637
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT189241.2094818159100162No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT63880.4082736123458668RNA PCR Primer, Index 11 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC62290.3981115108488423No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT52950.338417153627327TruSeq Adapter, Index 11 (95% over 23bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA51250.3275520136619548No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41880.2676659186763447Clontech Universal Primer Mix Long (96% over 26bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG32210.20586244604978662No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27550.17607918002706058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG23700.15147283363489422No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23480.150066756698199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG20980.13408860969029876TruSeq Adapter, Index 11 (96% over 26bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC19810.12661083689060146No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG19070.121881305376263No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA17440.11146355352711206No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG16590.10603098354442594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)