FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002295678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936109
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT506271.7242888462247146No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC190650.6493287544842511No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG156740.5338357669963888No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT145110.49422552091901223Illumina PCR Primer Index 10 (95% over 22bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA136350.46439011630698995No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT95890.3265886927222389Illumina PCR Primer Index 10 (95% over 24bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG85290.2904864907944494No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG80170.2730484460897058No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG79300.270085340837142No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC62200.21184499621778344No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC51780.17635585054914513No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT50330.17141734179487206No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT46270.1575895172829074No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC46230.1574532825586516No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA45740.15578440718651795No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAAA42460.14461315979754158No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC39890.13586007876410583No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT39320.13391873394346054No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT39170.13340785372750127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38940.13262450406303036Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC37130.12645988279045497No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC36530.12441636192661784No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG35930.12237284106278071No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT34690.11814956461085062No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT34400.117161862859996No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31560.10748919743783353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31200.10626308491953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC30520.1039470946071825No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT29460.10033687441440356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)