FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002295683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890768
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT239010.8268045031631732No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG230970.7989918250098245No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA188140.6508305059416736No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG137510.47568673791878147No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG108060.3738106966729948No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC88980.3078074753837043No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG88330.30555893797080913No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA67850.2347127130229752No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA67380.23308684750903566No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC59410.20551631953861396No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT58510.2024029600438361RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGT57120.19759454926856806No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT47720.1650772389897771No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT46810.16192928661172393No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC45160.15622146087129785No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT43900.15186275757860887No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC42350.14650086067093587No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC36500.12626402395487982No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT35400.12245880679459577RNA PCR Primer, Index 41 (95% over 24bp)
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT33240.11498674400712891No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAAA33120.1145716294078252No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC33110.11453703652454987No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG31700.10965943998273123No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29600.10239493449491623No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT29490.10201441277888781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)