FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214641
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG67343.1373316374783937No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA64302.9956997964042285No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG57682.68727782669667No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT49192.2917336389599376No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG46902.185043863940254No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT33471.559347934458002No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT33191.5463028964643288No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC29921.3939554884667889No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT24421.1377136707339233No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC22211.0347510494267171No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT21450.9993430891581757No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT20160.9392427355444672No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC20010.932254322333571No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC19650.9154821306274198No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT18330.8539840943715319No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT18030.8400072679497393No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT17650.8223032878154686No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC17100.7966791060421821No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT16650.775713866409493No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC15820.7370446466425333No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC15350.7151476185817248No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT15100.703500263230231No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC13640.6354797079775066No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT13600.6336161311212676No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC13540.630820765836909No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT12320.573981671721619No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT11600.5404372883093165No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT11390.5306535098140617Illumina PCR Primer Index 7 (95% over 22bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC10760.501302174328297No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT10510.4896548189768031No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA10450.48685945369244465No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT10050.4682236851300543No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA10030.4672918967019349No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC10020.46682600248787515No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA9660.45005381078172385No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA9470.4412018207145886No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA9470.4412018207145886No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA9390.4374746670021105No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT8890.41417995629912274No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG8640.40253260094762877No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT8110.3778402076024618No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA7730.3601362274681911No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC7620.35501139111353375No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7620.35501139111353375No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC7450.3470911894745179No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC6880.32053521927311185No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6860.3196034308449924No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT6660.3102855465637972No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA6510.30329713335290087No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT6460.3009676622826021No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT6040.28140010529209236No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA5810.270684538368718No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA5760.2683550672984192No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT5750.26788917308435944No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5740.2674232788702997No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT5730.2669573846562399No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC5640.2627643367297022No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC5460.2543782408766266No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT5430.2529805582344473No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA5370.25018519295008873No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC5100.23760604917047534No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT5060.2357424723142363No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC4780.2226974343205632No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC4690.21850438639402536No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA4540.21151597318312906No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT4270.19893682940351565No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA4200.19567556990509735No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA4180.19474378147697782No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG4170.1942778872629181No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC4090.19055073355044003No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC3990.1858917914098425No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT3960.1844941087676632No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG3910.18216463769736443No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT3850.1793692724130059No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA3840.17890337819894614No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG3770.17564211870052787No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG3620.16865370548963152RNA PCR Primer, Index 41 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC3590.16725602284745225No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACA3440.16026760963655592No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC3440.16026760963655592No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATA3430.1598017154224962No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT3410.15886992699437666No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA3380.1574722443521974No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG3330.1551427732818986No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG3320.15467687906783886No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATA3290.1532791964256596No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC3280.15281330221159983No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC3240.1509497253553608No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC3230.15048383114130104No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG3140.14629078321476324No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC3120.14535899478664374No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC3120.14535899478664374No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC3040.1416318410741657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCC3030.14116594686010595No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT3030.14116594686010595No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCT3000.13976826421792668No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA2980.13883647578980715No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT2910.13557521629138888No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC2900.13510932207732912No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG2890.13464342786326935No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC2880.1341775336492096No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA2870.13371163943514985No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC2870.13371163943514985No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA2820.1313821683648511No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA2780.12951859150861206No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTTTGCCGT2780.12951859150861206No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA2720.12672322622425353No Hit
GCGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCT2610.12159838986959622No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT2610.12159838986959622No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC2550.11880302458523767No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2540.11833713037117792No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2510.11693944772899864No Hit
GTGTATAAGAGACAGCTGAGGACGCTGCGGATGCTGTCTCTTATACACAT2490.11600765930087915No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT2470.11507587087275964No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC2460.11460997665869987No Hit
ATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCC2410.1122805055884011No Hit
GTGTATAAGAGACAGCTGAGGACGCTATGGATGCTGTCTCTTATACACAT2390.1113487171602816No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTTTGCCGT2380.11088282294622184No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGT2330.10855335187592305No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTC2310.10762156344780353No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCT2300.10715566923374378RNA PCR Primer, Index 7 (95% over 21bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATC2250.10482619816344502No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT2230.1038944097353255No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGA2230.1038944097353255No Hit
GAGATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCT2220.10342851552126574No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC2190.10203083287908647No Hit
AGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAG2190.10203083287908647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)