FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455521
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT292386.4185844340875615No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC136232.9906414852443683No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT72171.5843396901569848Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT42220.9268507928284316Illumina PCR Primer Index 10 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG41830.9182891677880932No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG30410.667587224299209No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT26360.5786780411880024No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT24360.5347722717503693No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT23450.5147951466562464No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG22380.4913055600071128No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC21390.46957220413548445No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA21320.46803550220516726No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT20590.45200989636043126No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC17660.38768794413429897No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG16510.36244212670766No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT14730.3233659919081667No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT14540.3191949438115915No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC14030.30799897260499515No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT13360.29329053984338815No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG13260.2910952513715065No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAAA13100.2875827898164958No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC13100.2875827898164958No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT12640.2774844628458403No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT12410.2724352993605125No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCT11460.25158005887763685No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG11230.24653089539230902Illumina PCR Primer Index 10 (96% over 25bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC11210.2460918376979327No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT11000.24148173190698125No Hit
CCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCT10480.23006623185319666No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTTTGCCGT10020.21996790488254112No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC9950.21843120295222396No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT9450.2074547605928157No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA9400.20635711635687484No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT9040.19845407785810096No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT8930.19603926053903112No Hit
TTTTAGCACTTACCTGTGACCACGAGACCCCATGTACTCTGCGTTGATAC8730.19164868359526785No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCC8600.18879480858182168No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT8530.18725810665150452No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA8290.18198941431898857No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT8240.18089177008304777No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC8050.17672072198647262No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA8030.1762816642920963No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC7970.17496449120896732No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT7640.16772003925175788No Hit
CCAGAGGGGAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCG7590.16662239501581705No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT7580.16640286616862887No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA7510.16486616423831174No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA7340.16113417383611292No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGT7020.15410925072609166No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT6850.15037726032389287No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC6700.1470843276160704No Hit
CCAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCC6510.14291327951949526No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT6330.1389617602701083No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA6180.13566882756228582No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC5840.1282048467578882No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA5800.12732673136913558No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5770.12666814482757105No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG5680.12469238520287759No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT5670.12447285635568942No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA5570.12227756788380777No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC5520.12117992364786695No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT5450.11964322171754979No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCG5440.11942369287036164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGC5370.11788699094004448No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCT5370.11788699094004448No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT5250.11525264477378651No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA5140.11283782745471668No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG5100.11195971206596403No Hit
GGTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAA5080.11152065437158769No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC5050.1108620678300232No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT5030.11042301013564687No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGG4990.10954489474689422No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT4970.10910583705251789Illumina PCR Primer Index 10 (96% over 27bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC4790.10515431780313092No Hit
GAGCTGAGGTGAAGATCCCCCATGTACTCTGCGTTGATACCTGTCTCTTA4770.10471526010875458No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT4650.1020809139424966Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTGT4640.10186138509530845Illumina PCR Primer Index 10 (95% over 22bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT4640.10186138509530845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)