FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710092
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG19910.2803862034778592RNA PCR Primer, Index 29 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGC15130.2130709823515826No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG8690.12237850870028111No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG8060.11350641888656682RNA PCR Primer, Index 29 (96% over 30bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT7630.10745086552165072No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG7340.10336688767089335RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)