FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA215.384615384615385TruSeq Adapter, Index 12 (96% over 29bp)
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT215.384615384615385TruSeq Adapter, Index 12 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA17.6923076923076925No Hit
TCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGGGGGGGGG17.6923076923076925Illumina Paired End PCR Primer 2 (95% over 23bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC17.6923076923076925No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC17.6923076923076925No Hit
GGCTGAGACCTCAGGAACATAATTGTCTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTAC17.6923076923076925No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC17.6923076923076925No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG17.6923076923076925RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG17.6923076923076925TruSeq Adapter, Index 12 (96% over 29bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)