FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG125.0TruSeq Adapter, Index 7 (96% over 28bp)
CATCTCCGAGCCCACGAGACATCATCTCCGGTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGGGG125.0Illumina Paired End PCR Primer 2 (95% over 22bp)
CATATGTACAATGTGACCACACACAGCCTGACATCTTTCACCTGTCAAGTCCAATTTAACCAGGAATTGGCATTT125.0No Hit
CATCCGCAGCGTCATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC125.0RNA PCR Primer, Index 29 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)