FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG125.0RNA PCR Primer, Index 32 (96% over 33bp)
TCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG125.0RNA PCR Primer, Index 32 (96% over 33bp)
TCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGG125.0RNA PCR Primer, Index 36 (96% over 29bp)
ATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGG125.0RNA PCR Primer, Index 37 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)