FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACGAGCCAACAAGACAACCAAAGCTATACAAAGACGACTACACCTAGAAAAAAAAAAAGGGGTCGGGTGG111.11111111111111No Hit
GTCTTTGTACTCTTTCCTGTGAATTGCTTTTTCCCTTTAACTTCCATCGTAGCAACTCGCACACGTGGGACCGAG111.11111111111111No Hit
CATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG111.11111111111111TruSeq Adapter, Index 7 (96% over 28bp)
GACCAGTACAGCAACTGCACCCCGTCCCACATCCCTTTCTCCAACAGGTCAGGATTTTACTGGAATGGCGTGGCT111.11111111111111No Hit
TCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGGGGGG111.11111111111111Illumina PCR Primer Index 6 (96% over 27bp)
TCAACAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGCCTTCTATTTCGACCGCGATG111.11111111111111No Hit
CAATAAAACAAAAATAAAACAAAAACAGACATTCTAATATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111.11111111111111No Hit
GACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC111.11111111111111No Hit
CATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG111.11111111111111TruSeq Adapter, Index 4 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)