FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCTCTAAGAGGTTTCTTCTAGAAACAGACAACTCAGACTCTTCCTCTCACTTCAGCAAAGAAGTTATTTTT114.285714285714285No Hit
CTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG114.285714285714285Illumina PCR Primer Index 6 (96% over 27bp)
CTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG114.285714285714285TruSeq Adapter, Index 25 (96% over 28bp)
TAATTCCTTAATGAAAGTTCTAGTTCCAGCTTGCACTGGTTTGGAACTGTCTCTTATACACATCTCCGAGCCCAC114.285714285714285No Hit
GAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGGGGG114.285714285714285RNA PCR Primer, Index 31 (96% over 29bp)
TCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGG114.285714285714285RNA PCR Primer, Index 36 (96% over 29bp)
TCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG114.285714285714285TruSeq Adapter, Index 4 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)