FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC12.2222222222222223No Hit
GACCATGAGTGCGATGAGCAGTGTCCGGCGCGAGGCACGGGCGGTGAGCGCCGCGATGACCGGCGCGCCGACCCA12.2222222222222223No Hit
TAAACAACGCACAGAACATCGGCCAAATACACCTAATAACCAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAA12.2222222222222223No Hit
GATGAGGAGATCAGCTTCAAGCTCTTCCACGAGTGCCTTCAGCTATTCTCCCTTGCCTTTTCCTATATATGTAGC12.2222222222222223No Hit
GGTATCAACGCAGAGTACATGGGGTGCCTCCAAGATGACAAAGAAAAGAAGGAACAATGGTCGCTGTCTCTTATA12.2222222222222223No Hit
GCTTAAGGACACCTTCCCTGCCCCCAGTGCAGTCATCAAATTCAGAGAGGATGCGGCAGTTCTGTACCTGTCTCT12.2222222222222223No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCC12.2222222222222223No Hit
CTCCAGGTCTGGTGACTCCGAATAGCCCTCTGAGTCTTTGCGCTGGTTGCGGTTGAACTCGCGGGCTTTGCCCCC12.2222222222222223No Hit
CAAACATGATCTGGGTCATCTTCTCGCGGTTGGCCTTGGGGTTCAGGGGGGCCTCGGTCAGCAGCACCTGTCTCT12.2222222222222223No Hit
ACATACGTACCTAGGTGTCTGCGGGTCCCCATTTTTGCACAGGAATGCCTGAGCAGCTTCCACTGATCCACCTTG12.2222222222222223No Hit
TCTCTATACTGGGCATTACTAGTGGGGAGGGGCAGCAAAGCTTGTATGGTATATTGAAAAGAACTAGAATTCATA12.2222222222222223No Hit
AACAATCTCTCAACAAAAAAAACAAAAAAAAACCCAATCAACTATGCATCTGACTATAATACAAAAAACACAGCA12.2222222222222223No Hit
CAGATCAAATGTTCAGGCATCAGAGAAGTGAAATTGACTCAGGGCATCTGCCTGGAGCAAAATAAGACCTCCAGC12.2222222222222223No Hit
CACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCTGCTGTGATGGCCACACTTCTCTTTCCAGG12.2222222222222223No Hit
GTGACACGGAGCTGGCACAGTTCCTCGCGATGGTCCCGGGTGGCGTCGCGCCCTTGCCAATCAACGAGGCGCAGG12.2222222222222223No Hit
GTCACCCTCACCCACTTCGCCCACTGCAACCTCCACCACCCCCTCGCGCTACAAGACTGAGCTATGTCGGACCTT12.2222222222222223No Hit
ATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTC12.2222222222222223No Hit
GAGTTAAGGCATCAGATTTTTTCTTCAGGAGGTTTCGACCTGTCTGTGCTCCCTTTAAACGAGCCTTCATGATGG12.2222222222222223No Hit
CTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG12.2222222222222223Illumina Single End Adapter 1 (96% over 27bp)
GGCTCGGACTGCCTTTGCTCTCGACACATTTGCTTGTGACATGACCAATTCTATGTCCTTAACTTCTACACCTGT12.2222222222222223No Hit
ATCAACAAAGACAACATGTCTCATATACACATCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA12.2222222222222223No Hit
ATCGGAGGGCGGTCGGAGGCTGGAAGAGAGTCCCCGGATCTGTTCCTGTCTCTTATACACATCTCCGAGCCCACG12.2222222222222223No Hit
TCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG12.2222222222222223Illumina Paired End PCR Primer 2 (96% over 28bp)
ACAGCCCACTGTCCCCCAGCTGGGCTAGATGACCTCAAAGTTTGGAGAGTCAGGGACAGGGTCCAGGGATGGGAA12.2222222222222223No Hit
ATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA12.2222222222222223No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCG12.2222222222222223No Hit
TATTTATTTGTTACTCAAAAAATCTTATTTCTGACTGGATTCAGACTTAGAAGTAGAAGCTCGCAGAGAGGAAAG12.2222222222222223No Hit
GACTTAGGGCAATGAGGAAGATCTTAAAACCAGTCCAAGCCTGAGACAGAGGCCCTGCTGTCTCTTATACACATC12.2222222222222223No Hit
CTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG12.2222222222222223RNA PCR Primer, Index 1 (96% over 28bp)
CAGGCTTATGCCCGTATAGAGGGGGATATGATAGTCTGCGCAGCGTATGCACACGAACTGCCAAAATATGGTGTG12.2222222222222223No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC12.2222222222222223No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAG12.2222222222222223No Hit
CAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAACGATCACGGCAGTGTAGATCAGAGCATCGGACAGGTACA12.2222222222222223No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGAAGGAGATTCAAAACAAGGAAAAAAACAAGAGAGGCGCACC12.2222222222222223No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT12.2222222222222223No Hit
ACCCACAGGTTAGTTCAGTCAAAGCAGGCAACCCCCTTGTGGGCACTGACCCTGCCACTGGGGTCATGGCGGTTG12.2222222222222223No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGCTGTCTCTTATACACAT12.2222222222222223No Hit
CCACTCCACTCTTGCCCTCAACCCCTTTCAACCAAACATTATCACCCACCTTAAGAAATTAAAAAATAATTCTGT12.2222222222222223No Hit
GGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATCGGCCGTATGCGGCGGTTGTTCTGT12.2222222222222223No Hit
GTGAGCAGACTTAACTAAAAGGGATTGTGATGATCTACTCCAACAATTTCATTTTTAAAATTAGGAAACGAGGCC12.2222222222222223No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA12.2222222222222223No Hit
GGCAAGCTGAGTCCAAAAGGCAGTCAGCAAAGGGTGGTGGGATTATCATTAGTTCTTATAGGTTTGGGATCTGTC12.2222222222222223No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG12.2222222222222223TruSeq Adapter, Index 6 (96% over 28bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC12.2222222222222223No Hit
GCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGCTGTCTCTTAT12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)