FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGC16.25No Hit
AACTATGGGTGAGCATGATCGAGCTAGTGGATCAGATATCCAGGTAGTGAACTGCTATCAGACTCACGTAACGCA16.25No Hit
GAAGATGTTACAACGATTATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAAGACGCTA16.25No Hit
GCCCAGGCTGGAGTGTAGTGGTACAGTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATC16.25No Hit
TCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCATCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 31 (96% over 28bp)
GTATGGAATCCAGAAGGACCGAGTGGATAAGAGCGCTGTCGGCTTCAATGAAATGGAGGCCCCGACCACAGCTTA16.25No Hit
TCTCCGAGCCCACGAGACATCATCTAATCTCGTATGACGTCTTCTGCATGAAAAAAAAAAGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 29 (95% over 24bp)
TCCGCGCCCACGAGACCCACGTATATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGTGGGGGGGGGGGGGGG16.25TruSeq Adapter, Index 6 (96% over 26bp)
AAATAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTAAACACATCACAGAGAAAAAGAAAAAGCAGCAA16.25No Hit
CTGCTTTCCCATGTACTCTGCGTTGATACCACATGTAACGATTTACACTGACGTAAAACACCAGAGTTCCCATAA16.25Clontech SMART CDS Primer II A (95% over 21bp)
CATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG16.25RNA PCR Primer, Index 32 (96% over 33bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC16.25RNA PCR Primer, Index 29 (95% over 21bp)
TCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 31 (96% over 29bp)
AAATTTAGCAAAAGAATGGGTGAATGAAAGAATACAATTTTCTGGCCAGGTGTGGTGGCTCACGCCTGTAATCCT16.25No Hit
GTAGCGGCGTCGGCCCTGTTCCGTTTTTGTGTGACGCTGGTCAGTACTTTTCCGTCTGCTGTTTTCGTCAGAGAT16.25No Hit
CTCCACGAACAGTGCGCTGAGCTACCTTCAGAGCCCCATTACGACCTCTCCCAGCTGCTGAAAGGCCACGGGAGG16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)