FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAAACCAGAGACAGAGGGAAGCTGAGCGAAAATAGACCTTCCCGAGAGAGGAGGAAGCCCGGAGAG12.564102564102564No Hit
GTATCAACGCAGAGTACATGGGGGATCACCTGAGGTTGGGAGGTTGAGACCAGCCTGACCAACATGGAGAAACCC12.564102564102564No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12.564102564102564No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTCTGTCTCTTATAC12.564102564102564No Hit
GTATCAACGCAGAGTAAATGGGTCGTCGGCAGCGTCAGACGTGTATAAGCTGTCTCTTATACACATCCTCCGAGC12.564102564102564No Hit
CCTTTCCATCGAGCCACTGGGCGCCCTGAAACAGGCGCCTGGGAAGGCGGCAAGGCGTCGTTCTGCCAGCCCGGA12.564102564102564No Hit
CCCCCAGTCACCACCACCGCAGAGTCTGACTTTGAACCACAGACCAAGTCCCGCTCCAGCAGCTCAGCACTGGCC12.564102564102564No Hit
CATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG12.564102564102564Illumina PCR Primer Index 6 (96% over 27bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT12.564102564102564No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA12.564102564102564No Hit
GTTACCATCTCCCCAGGGAAACGGCTTGGTCCTGATGCGGAGATGGGGGTAGGCGATGAACTCGGGTCTCTCGTG12.564102564102564No Hit
GCTGTGGCTGGGTGCCCCCACCCCCTGGCTTCGCACCGCTGGCTCCCCGCCTGGGCCCTGAGCTGTCACCCTCAC12.564102564102564No Hit
GATGGGATGAGAAAGGACAGGCGGCAGGCGGAGGGGCCTGCCTGATGGGTCCCGCACTGTCACCTGGAAGCAGAG12.564102564102564No Hit
CACTTACCTGTGACCACGAGACCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA12.564102564102564No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGG12.564102564102564No Hit
CTCTAGAGGAAGGTGGTCCTGACATGGTTTGGGAGCAGAGAAGAAAGGAGGTTGAGGCTGTCTCTTATACACATC12.564102564102564No Hit
GGTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACGACGTCATCACGTATG12.564102564102564No Hit
TCCATGGAGTGGGCTTCAAGAAGCGTGCACCTCGGGCACTCAAAGAGATTCGGAAATTTGCCCTGTCTCTTATAC12.564102564102564No Hit
CCTCCCCTCAAAATGAAGGTGTCTAAAAACAGTGATTCATCAGCATTGCTTTAAATAATTGTTTGGTTAAAAGTT12.564102564102564No Hit
TCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG12.564102564102564Illumina PCR Primer Index 7 (96% over 27bp)
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT12.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GAGCTGATGGAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTGCTGCCTTCGTGAAGGAGATC12.564102564102564No Hit
GTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG12.564102564102564No Hit
CAGCAGGGCTGTGGCTACCATGTTCTCTCGCGCGGGTGTCGCTGGGCTGTCGGCCTGGACCTTGCAGCCGCAATG12.564102564102564No Hit
ACCTAGAAGAGAGGCGGGTCAAAGAAGTAGTGAAGAAGCATTCTCAGTTCATAGGCTATCCCATCACCCTTTATT12.564102564102564No Hit
TCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG12.564102564102564Illumina PCR Primer Index 6 (96% over 27bp)
CCTTACCATGGTCACTGCTGTCCTTTCACTCTGTCATTCATTTATCAGACTTCTTCATCAATTCCGTCTCTGTCT12.564102564102564No Hit
TCGATGATGTCATGAGGATGGCGGCCATCAACATTACAGCCCACTGACTGGGCAGTCCCCAGGATCTCTTTAATG12.564102564102564No Hit
ACAGAATACTGGCCATGGTGCAACTTCTGGTTTTGGCAGTGCTTTTACAGGACAACAAGGATTTGAAAATTCAGG12.564102564102564No Hit
GGGTAGGGGAAGACCACCGAGGGGTCGATGCACTCTGAGGCGGCGGCGCTCAGATCCTGCAGGTACAAGCTGGAG12.564102564102564No Hit
GTGCTTTTAGTGCTGCTTCCTCCTGAAGGAACATCCTTCTGTAAGCCTTGCTTTTCCTCCTGTAGGCTGGCAGAG12.564102564102564No Hit
GGATATGATAGTCTGCGCAGCGTATGCACACGAACTGCCAAAATATGGTGTGAAGGTTGGCCTGACAAATTATGC12.564102564102564No Hit
GGACTGGGAGAGAGTCTCCAACATGACCACGCAGATCTGTTTGACACACTCAATGATGGATCTGTCTCTTATACA12.564102564102564No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG12.564102564102564Illumina PCR Primer Index 7 (96% over 27bp)
ACAGCTGGATGGCTGTCATCCGCTCTCAGAGCAGTACCCAGCCTGCTGAGAAAGATAAGAAGAAACGTAAAGATG12.564102564102564No Hit
CAATCGTTAATATACCATCGTCCAAGCTTCTTCTGGGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT12.564102564102564No Hit
GGTATCACCAGCAGGAGGTACTTTGGATGACAAACCAAAGGAACAACTGCAGAGGAGTCTCCCTAAAGCAACTGA12.564102564102564No Hit
CTCTAACAAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTGGGAAAGC12.564102564102564No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCG12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)