FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCCA15.0No Hit
CAGTCTCTGAGGAAGCCACAGAAGTTCACATGATGGAGGGGGACCCAGACACACTGGCCGAACTTCTGATCCTGT15.0No Hit
AGGTAGCCCTGGCCAATCTTATAAATCTTCTTGTTGATCTCAGTGCGGTGATGGTAGCCTTTCTGCCCAGCGCGT15.0No Hit
CGTCAGAAGTGTATAAGAGACAGCATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA15.0No Hit
GCGTCAGATGTGTATAAGAGACAGTCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGA15.0No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATC15.0No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC15.0TruSeq Adapter, Index 11 (95% over 21bp)
AGTGACTGTAGATTTGGATTACTGCTCTCTGCATCTTCCAGTTTTTCTGTGTTATCTTCCCAATTAATGTGGAAT15.0No Hit
TCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0Illumina PCR Primer Index 6 (96% over 27bp)
CCAAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACGAAAAAAACCAACCAAAAAAAAAAAAAA15.0No Hit
ATCTCGCTTCCCCACCACCACCTTCCCGTGAGCCCCGAGCCCTCAGGCAGTTGCTGTGACCAACAGGATGTGTCA15.0No Hit
TCTCCGAGCCCACGAGACACCATTCATTCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAGGGGGGGGGGGGGGG15.0TruSeq Adapter, Index 4 (96% over 26bp)
CGTCGGCCTCGTGCGCCAACAACGCCTTCGCCTTCGGTCCGGAGCTCAGCAGCCTCATCACGCCGCTCGCCATCC15.0No Hit
AAATAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAACCCAGGCAAAAAAAAAAAAAAAACCACCCCCG15.0No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACCTGTCTCTTATACACATCTCC15.0No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAACAACCACATG15.0No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC15.0No Hit
ACCTGGAACCTCTGGGAGGGCTGCAGAGTAAGTGCCGCCTCTGCGCTCCGACGGAGGCACGAGGCCTGTGGAGTA15.0No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCAGCTTGAAAAAAAAAAGGGGG15.0Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0RNA PCR Primer, Index 45 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)