FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138218
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA127349.21298239013732No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA61694.463239230780362No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC47243.4177892893834376No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC29622.1429915061714104No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC28192.0395317541854174No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC27631.9990160471139793No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC25021.8101839123703136No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC24781.7928200379111259No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC21141.5294679419467798No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG17831.2899911733638165No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC16561.1981073376839486No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC16561.1981073376839486No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT16351.1829139475321593No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC14781.0693252687783068No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG14531.0512378995499863No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC13951.0092752029402827No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA12730.9210088411060788No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC11560.836359953117539No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC11450.8284015106570779No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC11350.8211665629657497No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC10890.78788580358564No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT10400.7524345598981319No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT9790.70830137898103No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9370.6779145986774515No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAT9330.6750206196009203No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8690.6287169543764198No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG8490.6142470589937635No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT7850.5679433937692631No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC7840.5672198990001303No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT7310.5288746762360909No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG6960.5035523593164422No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC6890.4984878959325124No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTA6880.4977644011633796No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC6870.4970409063942468No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA6310.45652519932280894No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA6210.4492902516314807No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA6120.4427787987092853No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATA5030.36391786887380806No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACA4970.3595769002590111No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT4890.3537889421059486No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGT4840.3501714682602845No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA4820.34872447872201884No Hit
GTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA4710.3407660362615578No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT4690.3393190467232922No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT4580.33136060426283115No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA4140.29952683442098715No Hit
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGA4060.29373887626792455No Hit
GACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGA4000.2893979076531277No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA3960.2865039285765964No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATAC3920.28360994950006513No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACAC3840.27782199134700253No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT3830.2770984965778697No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA3490.2524996744273539No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCC3430.24815870581255697No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC3380.24454123196689287No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG3350.24237074765949443No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCAC3330.24092375812122876No Hit
GTCCAGGGTGGACATGGGCACGCTGCTGTCTCTTATACACATCTCCGAGC3320.24020026335209596No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT3250.23513579996816622TruSeq Adapter, Index 1 (95% over 22bp)
GGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT3140.22717735750770524No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA3080.22283638889290833No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA3080.22283638889290833No Hit
GGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT2980.21560144120158012No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT2930.21198396735591604No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATA2910.21053697781765038No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCT2910.21053697781765038No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2880.2083664935102519No Hit
GTCCAGGGTGGACATGGGCACGCCTGTCTCTTATACACATCTCCGAGCCC2880.2083664935102519No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT2870.20764299874111908RNA PCR Primer, Index 1 (95% over 24bp)
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCG2830.20474901966458783No Hit
ATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACAT2810.20330203012632217No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTC2770.20040805104979093No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGCTGTCT2750.19896106151152526No Hit
GAGATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCT2560.1852146608980017No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG2550.18449116612886887No Hit
GGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC2480.17942670274493916No Hit
AGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTC2460.17797971320667352No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA2410.17436223936100942No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC2350.1700212707462125No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG2340.16929777597707968No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACCTGTC2320.16785078643881407No Hit
GTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTC2220.16061583874748586No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT2210.15989234397835303No Hit
GTCCAGGGTGGGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAT2190.1584453544400874No Hit
GTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTC2160.15627487013268895No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC2140.1548278805944233No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT2140.1548278805944233No Hit
AGCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTG2120.15338089105615765No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTC2030.14686943813396228No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT1990.143975459057431No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCT1930.1396344904426341No Hit
AGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCG1860.13457002705870436No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCC1830.13239954275130592No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGACTGTC1780.1287820689056418No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACACTGTCTCTTATAC1730.1251645950599777No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTCTGTCTCTTATACACATCTC1720.1244411002908449No Hit
GATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTA1690.12227061598344643No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATC1690.12227061598344643No Hit
GTCCAGGGTGGGCATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG1680.12154712121431363No Hit
TCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTC1660.12010013167604798No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCCTGTCTCTTATACACATCTC1610.11648265783038389No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCTGTCTCTTATACACAT1610.11648265783038389No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCTGTCTCTT1580.11431217352298542No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGCTGTCTCTTAT1530.11069469967732133No Hit
CCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACAT1490.10780072060079006No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTC1480.10707722583165724No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG1440.10418324675512596No Hit
GTCCAGGGTGGACATGGGCACCTGTCTCTTATACACATCTCCGAGCCCAC1430.10345975198599314No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCT1420.10273625721686033No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCCTGTCTCTTATACACATC1420.10273625721686033No Hit
AGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTC1390.10056577290946188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)