FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42448
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA25045.898982284206559No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC22795.368921975122503No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA14863.500753863550697No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC7211.6985488126649078No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6131.444119864304561No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA4861.1449302676215605No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC4401.0365623822088201No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC4351.0247832642291745No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC4251.0012250282698831No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC4240.998869204673954No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC4140.9753109687146627No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC4000.9423294383716547No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG3710.8740105540897097No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC3620.8528081417263476No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC3340.7868450810403317No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT3040.7161703731624576No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG2910.6855446664153788No Hit
GTTTCCAGGTCAAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2360.5559743686392763No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG2330.5489068978514888No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG2320.5465510742555597No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA2290.5394836034677724No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG2280.5371277798718432No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCT2250.5300603090840559No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTA2250.5300603090840559No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2220.5229928382962684No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG2140.5041462495288352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC2130.5017904259329062No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA1960.46174142480211083No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG1860.4381831888428195No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG1840.4334715416509612No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT1790.4216924236713155No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT1770.41698077647945725No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT1750.41226912928759896No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACC1670.39342254052016584No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTC1620.38164342254052014No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT1610.379287598944591No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT1480.34866189219751226No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA1410.33217112702600826No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT1390.32745947983415No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1360.3203920090463626No Hit
GGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT1330.3133245382585752No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT1310.3086128910667169RNA PCR Primer, Index 46 (95% over 23bp)
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA1230.28976630229928385No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATAC1220.2874104787033547No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTC1210.28505465510742556No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA1210.28505465510742556No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTG1200.28269883151149644No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1200.28269883151149644No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1180.27798718431963815No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCC1150.27091971353185074No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC1150.27091971353185074No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACACAT1140.2685638899359216No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1120.2638522427440633RNA PCR Primer, Index 46 (95% over 21bp)
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATC1090.2567847719562759No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT1070.2520731247644176No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCAC1000.23558235959291368No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA990.23322653599698456No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC970.22851488880512627No Hit
GCGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTT970.22851488880512627No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC960.22615906520919715No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTCTGTCTCTTATACACATCTCCG960.22615906520919715No Hit
AAAGTTGAAATGTCAGGGTCACGTCCCTGTCTCTTATACACATCTCCGAG940.22144741801733886No Hit
GTTTCCAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG940.22144741801733886No Hit
GATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGAT930.21909159442140974No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACAC930.21909159442140974No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG930.21909159442140974No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA920.2167357708254806No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC900.21202412363362233No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA890.20966830003769318No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC860.20260082924990577No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT850.20024500565397663No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT850.20024500565397663No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT840.1978891820580475No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTCTGTCTCTTATACACATCTC840.1978891820580475No Hit
AAAGTTGAAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG830.19553335846211836No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT820.19317753486618922No Hit
GTTTCCAGGTCAAGGGTCACGTCCTGTCTCTTATACACATCTCCGAGCCC820.19317753486618922No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT800.18846588767433095No Hit
GTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT770.18139841688654354No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACACTGTCTCTTATAC760.1790425932906144No Hit
GTTTCCAGGTCAAGGGTCACGTCCCTGTCTCTTATACACATCTCCGAGCC760.1790425932906144No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATA750.17668676969468525No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT740.17433094609875613No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA740.17433094609875613No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTG680.1601960045231813No Hit
CTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG660.15548435733132301No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA650.1531285337353939No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT650.1531285337353939No Hit
CCCTTGACCTGGAAACCTGTCTCTTATACACCTGTCTCTTATACACATCT640.15077271013946475No Hit
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACG630.1484168865435356No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT630.1484168865435356No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT630.1484168865435356No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA620.14606106294760648No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACCTGTC620.14606106294760648No Hit
GTCCAGGGTGGACATGGGCACGCCTGTCTCTTATACACATCTCCGAGCCC610.14370523935167734No Hit
GTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA600.14134941575574822No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC580.13663776856388993No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATCTGTCTC580.13663776856388993No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCG580.13663776856388993No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT570.1342819449679608No Hit
GTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTC570.1342819449679608No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGCTGTCTCTTATACACATCT570.1342819449679608No Hit
GTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCC560.13192612137203166No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT560.13192612137203166No Hit
AGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTC550.12957029777610252No Hit
GTTTCCAGGTCAAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA550.12957029777610252No Hit
TTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA530.12485865058424425No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC520.12250282698831512No Hit
TGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCC520.12250282698831512No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA520.12250282698831512No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG510.12014700339238599TruSeq Adapter, Index 4 (95% over 23bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTC500.11779117979645684No Hit
GTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTC500.11779117979645684No Hit
GGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT500.11779117979645684No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC490.11543535620052771No Hit
GCGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCC480.11307953260459858No Hit
GATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTG470.11072370900866943No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTA470.11072370900866943No Hit
GTACTACCGACCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC470.11072370900866943No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTGTCTCTTATACACATCTCCGAGC470.11072370900866943No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGCTGTCTCTTAT470.11072370900866943No Hit
AGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTA460.1083678854127403No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCT460.1083678854127403No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCTGTCTCTTATACACAT460.1083678854127403No Hit
GTCCAGGGTGGACATGGGCACGCTGCTGTCTCTTATACACATCTCCGAGC460.1083678854127403No Hit
AGCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTG460.1083678854127403No Hit
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGA450.10601206181681117No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC450.10601206181681117No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG440.10365623822088202No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT440.10365623822088202No Hit
GTGTATAAGAGACAGATCAAGAAGGACGTGACCCTTGACCCTGTCTCTTA430.10130041462495289No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGCTGTCT430.10130041462495289No Hit
GTACTACCGACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGA430.10130041462495289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)