FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965873
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA582926.035161972640295No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG398854.1294248829815094No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG335753.476129884570746No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG202652.09810192437308No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC189401.960920328034845No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA161371.6707165434793187No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT147941.5316713480964887No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC147711.5292900826506175No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT142191.4721397119497075No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT136971.418095339656456No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC131101.3573213041466115No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC113251.1725143988909514No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC112151.1611257380628717No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC106511.1027329679988984No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT103901.0757107818522726No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT93410.9671043708644924No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT87550.9064338686349033No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC83600.8655382229340711No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT77560.8030041216598869No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT75790.7846787310547039No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT75580.7825045321693431No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC70980.7348792232519182No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT68740.7116877684747374No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG61930.6411816046208973No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC59810.6192325492067797No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA59620.6172654168819296No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA55690.5765768377416078No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT51580.5340246595566912No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT50640.5242925312126957No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC46030.47656368901501545No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC44040.4559605662442164No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT43170.4469531708620077No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT41650.43121611226320644No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA41570.43038784602116426No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG39620.4101988563713863No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA39480.40874939044781244No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA38660.40025966146688025No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT38580.399431395224838No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC38410.39767132946049844No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT37620.389492200320332No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC37080.3839014031865473No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT36010.37282334219923324No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA35340.36588661242213No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT35100.3634018136960035No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT31770.32892523137099805No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA31200.32302383439644755No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT30560.3163977044601102RNA PCR Primer, Index 1 (95% over 24bp)
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT30260.313291706052452No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT30100.3116351735683677TruSeq Adapter, Index 1 (95% over 22bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA28420.29424158248548204No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC28410.2941380492052268No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC27800.28782251910965523No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAT27170.2812999224535731No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC26830.27777979092489385No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG25950.2686688622624299No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC25830.2674264628993667No Hit
GTCCAGGGTGGACATGGGCACGCCTGTCTCTTATACACATCTCCGAGCCC24750.25624486863179735No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG24400.25262120382286285No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA24320.25179293758082066No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC23970.24816927277188616No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCT23860.24703040668907816No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC23830.24671980684831235No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA23150.23967954379095385No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT21860.22632375063802385No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT21790.22559901767623694No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA21490.22249301926857878No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA20700.21431389012841234No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC20490.21213969124305163No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACC20110.20820542659335128No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT20100.208101893313096No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT19120.1979556318480794No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG19050.1972308988862925No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT18770.1943319670391449No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA18720.19381430063786853No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCC18630.1928825011155711No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT18610.19267543455506053No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA17890.185221038376681No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG17650.18273623965055447No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATC17510.18128677372698068No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG16780.17372884426834584No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT16580.1716581786632404No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG16220.16793098057405065No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA16090.1665850479307321No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC16050.166170914809711No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT15910.1647214488861372No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTG15900.16461791560588193No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCG15610.16161545047847906No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT15610.16161545047847906No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC15460.16006245127464996No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA14870.15395398773958896No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTC14560.15074445605167552No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC14280.14784552420452793No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG14160.14660312484146468No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC14140.14639605828095412No Hit
GTCCAGGGTGGACATGGGCACGCTGCTGTCTCTTATACACATCTCCGAGC14030.14525719219814615No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC13960.1445324592363592No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG13450.1392522619433404No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT12930.13386853137006624No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT12700.131487265924195No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT12610.13055546640189758No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC12530.1297272001598554No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC12460.1290024671980685No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC12390.12827773423628158No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12360.12796713439551577No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC12270.12703533487321833No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACA12060.12486113598785761No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT11990.12413640302607071No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATA11940.12361873662479436No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA11840.12258340382224164No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA11730.12144453773943364No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCT11380.11782087293049914No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC11320.1171996732489675No Hit
AGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTC11210.11606080716615953No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGCTGTCT11200.11595727388590425No Hit
GACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCC10970.113576008440033No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTC10960.11347247515977775No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG10760.1114018095546723RNA PCR Primer, Index 40 (96% over 26bp)
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC10750.11129827627441703No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC10680.11057354331263014No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC10540.10912407738905633No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGA10500.10870994426803525No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC10490.10860641098777997No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA10420.10788167802599306No Hit
GAGATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCT10370.1073640116247167No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC10250.10612161226165345No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10220.10581101242088764No Hit
AGCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTG10190.10550041258012181No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTG9800.10146261465016622No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTC9780.10125554808965567No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATA9660.10001314872659242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)