FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC67123.274366978841485No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT49717.238987166146373No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2478.567464446756851No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG652.2545959070412764No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC481.664932362122789No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC481.664932362122789No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG471.6302462712452306No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC431.4915019077349982No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA421.45681581685744No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT351.2140131807145336No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG280.9712105445716268No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC250.8671522719389525No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC230.7977800901838363No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT210.72840790842872No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT210.72840790842872No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG190.6590357266736039No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG180.6243496357960457No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG160.5549774540409296No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC160.5549774540409296No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA140.4856052722858134No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA140.4856052722858134No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA140.4856052722858134No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC130.4509191814082553No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC130.4509191814082553No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG120.41623309053069724No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGT120.41623309053069724No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT120.41623309053069724No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT100.346860908775581No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC90.31217481789802287No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC90.31217481789802287No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT90.31217481789802287No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG80.2774887270204648No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACG80.2774887270204648No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG70.2428026361429067No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT70.2428026361429067No Hit
CAATCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.20811654526534862No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG60.20811654526534862No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG60.20811654526534862No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG60.20811654526534862No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC60.20811654526534862No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG60.20811654526534862No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG50.1734304543877905No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGTT50.1734304543877905No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.1734304543877905No Hit
CAAGCAATCAAGGCCAGTCTCTATACACATCTCCGAGCCCACGAGACGTT40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG40.1387443635102324No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCACACGAGACGT40.1387443635102324No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAA40.1387443635102324No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACG40.1387443635102324No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTA40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC40.1387443635102324No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC30.10405827263267431No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGAC30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACG30.10405827263267431No Hit
CAAACAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCACATCTCCGAGCCCACGAGACG30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC30.10405827263267431No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGAC30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACACCTCCGAGCCCACGAGAC30.10405827263267431No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA30.10405827263267431No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACGT30.10405827263267431No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACG30.10405827263267431No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAACCCACGAGACGT30.10405827263267431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)