FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5045
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC117623.310208126858274No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAT81416.13478691774034No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4789.474727452923688No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1022.0218037661050547No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC981.9425173439048562No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG641.2685827552031714No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC591.1694747274529236No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG561.110009910802775No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA551.0901883052527255No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT500.9910802775024776No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC480.9514370664023787No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG430.8523290386521309No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT410.8126858275520318No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC390.7730426164519326No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG360.7135777998017839No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG360.7135777998017839No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGT330.6541129831516352No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC300.5946481665014867No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT290.5748265609514371No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA220.4360753221010902No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC220.4360753221010902No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT220.4360753221010902No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG210.41625371655104065No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG180.35678889990089196No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG170.33696729435084244No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC170.33696729435084244No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG130.2576808721506442No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA130.2576808721506442No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG130.2576808721506442No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.19821605550049554No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG100.19821605550049554No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC90.17839444995044598No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT80.15857284440039643No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA80.15857284440039643No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG80.15857284440039643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC80.15857284440039643No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG80.15857284440039643No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTA80.15857284440039643No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG70.13875123885034688No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACA70.13875123885034688No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAT70.13875123885034688No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC70.13875123885034688No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT70.13875123885034688No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACA70.13875123885034688No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACA60.11892963330029734No Hit
CAACCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGAGACA60.11892963330029734No Hit
ACACCAGCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG60.11892963330029734No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC60.11892963330029734No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT60.11892963330029734No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT60.11892963330029734No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC60.11892963330029734No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC60.11892963330029734No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACA60.11892963330029734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)