FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687602
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT536023.1762228297904365No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC195991.16135202494427No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT181181.073594366444221RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT111520.6608193164028011RNA PCR Primer, Index 47 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG104430.6188070409966331No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC87560.5188427129145379No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA76600.45389849028384655No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG72100.4272334353716102No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT71340.42273000387532134No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT64520.38231763176388744No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT59790.3542896962672478No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA59110.350260310191621No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC57080.33823140764232323No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG46940.2781461505734172No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC44200.26191009491574435No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG44110.2613767938174996No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT43620.25847326561594497No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC41950.24857756745962617No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT41550.24620734035631625No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT40220.2383263352378108No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT38980.2309786312175501No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC36670.2172905696959354No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT36530.21646099020977694No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA34360.2036025081743207No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT30910.1831592994082728No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC30360.1799002371412217No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC30340.1797817257860562No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG29820.1767004305517533No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT29080.17231551041062998No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT28390.16822686865742043No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG27910.16538259613344852TruSeq Adapter, Index 9 (95% over 24bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA24740.14659854633971753No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT23990.14215437052101146No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT23890.141561813745184No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC23490.1391915866418741No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT23410.1387175412212121No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC23010.1363473141179022No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA22000.1303624906820447No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT21130.12520724673234565No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA21050.12473320131168367No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT20410.12094083794638784No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA19440.11519303722086131No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA19420.11507452586569582No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT18540.10986002623841404No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT18220.1079638445557661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)