FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924212
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT234681.2196161337732017No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC84650.4399203414176816No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT76720.39870866619686396RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT45670.2373439101304846RNA PCR Primer, Index 47 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG39740.2065261000347155No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC31430.16333959044013863No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA30030.15606388485260458No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG29770.15471268238634828No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT25530.13267768832124527No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT22960.11932157163555782No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT22470.11677507467992092No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC20110.10451031383236359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)