FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409285
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC37410.26545375846617253No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG29810.21152570274997604TruSeq Adapter, Index 11 (96% over 30bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG14410.10225043195663049No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC14280.10132797837201132TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)