FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373549
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGC56050.4080669855971647No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG29180.21244236645361758Illumina PCR Primer Index 11 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC17300.12595109457325512No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC15690.11422963432684236No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC13860.10090648386042289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)