FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773172
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC62150.35050181257091806No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG36220.2042667039632929TruSeq Adapter, Index 6 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC23550.13281283485189255No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC19510.11002880713207742No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC18830.10619387177329667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)