FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGCAGGCAACGCAAGATGGCGCTTCTGAAGGATGCGCTGAACCGTGCAGACGAGCGAAGGCTCAAGAGGCTG16.25No Hit
TCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25Illumina PCR Primer Index 2 (96% over 28bp)
AGACAGGAGTGCAGTGGCACAATATTGGCTCATTAAGTCTCCATCTGGGATCAAATGATTTTCCCACCTCAGTCT16.25No Hit
GGATGCGCCGGTACGCCGCCAGGTAGGGTTTGTGGCGGAAGGCCTTGCCGCAGTCGGGACACACGAAGGGCCGAT16.25No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCT16.25No Hit
CCCTGGACCACATAAATCATACCAGTCTCCATGTTAGGTGGATTAAGGTAAGTATGTGGATTTCTAATGTAACCA16.25No Hit
GGGAGATAGTGAGGGGAGGGCACGCTTGTCTTCTCAGGTATCTTCCCCAGCATTGCTCCCTTACTTAGTATGCCA16.25No Hit
CCTCCATAGCGCCAAAGCTGCTCTCGTGGTCGCCACAGTAACCCCGGCCCTGTCTCTTATACACATCTCCGAGCC16.25No Hit
GGGAGATAGTTCTCATAAAACCAAGACTATTTCGCAAACTTCAGCACCTCTCTGGGATGAGAGTGCCTCAATACT16.25No Hit
CCTCAACACCACATGAGCATATCTTCGGCCCACACCCTTAATGGCAGTGATGGCAAAGGCTATTTTCCGCCGCCC16.25No Hit
CAAAAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAATGCAGTCTACAGCTAGAAAAAAAAAAAAGGC16.25No Hit
AATCAAGCATTACAAAAAAGAGCAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.25No Hit
CAAAAATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACAAATACACAAATAAAAAAAAACGAGACC16.25No Hit
CCCAGTTAATCGACCCATAAACTCCATACAAATAGACATCATCTCAAACCTAATAAAAAAAAAAAAAAAAAAAAA16.25No Hit
GGCCAGATAGGTCAGCGTGGCGGCAGACAGATCGGCGAGATCCGTCGGATTCATCCGCATCTCGTTCCACATCTT16.25No Hit
TATCAAATAATATAAAATAAAGTATATTCAAAAAACCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.25No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)