FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTAT18.333333333333332No Hit
GCCTCTGGGAGGGTCTCTTCTGTGACGCCATCACATCCAGGCTCTCGGAGGTGGCTGCACTGTCTTCTTGGATGG18.333333333333332No Hit
GTCCAGGCTGGTCTTGACCTCCTGGCCTCAAGTGATCTTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18.333333333333332No Hit
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC18.333333333333332No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTT18.333333333333332No Hit
GGGCCGGCGCGCCCGTCTACATGGCTGCGGTCCTCGAGTATCTGACCGCCGAGATCCTGGAGCTGGCGGGCAACG18.333333333333332No Hit
CTACTTTACAGAGGAGGAGACCGAGACACAGAGAAGTTGGGTCGCTTACCCAAAACTACACAGCTAGGAAATGGC18.333333333333332No Hit
GTCCGGTTCGTTGGGTGCGACCTTCACCGACCGTGAGCAGCCAATGCAGCGGCACATCGAAGCACTCGCCGGGTT18.333333333333332No Hit
GTACATGGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCTCCTAAAGCCTGTCTCTTAT18.333333333333332No Hit
CTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAAGCAGGTGTGCGCCTCCTGCCACAGCAT18.333333333333332No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG18.333333333333332RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG18.333333333333332Illumina PCR Primer Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)