FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCTGAGGGACTCATCCTGGGCTACAATC18.333333333333332No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAAT18.333333333333332No Hit
ATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGG18.333333333333332RNA PCR Primer, Index 36 (96% over 29bp)
ATCAACGCAGAGTACATGGGCAAGCAGAAGACATCTCCGAGCGCACGAGACGTGCATTCATCTCGTATGCCGTCT18.333333333333332RNA PCR Primer, Index 45 (100% over 21bp)
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACGATGAAAAAGAAAAAT18.333333333333332No Hit
ATGATGAGCAGTACGCTTGGGAGTCCTCAGCAGGGGGAACATTCACAGTGAGGACAGACACAGGTGAACCTATGG18.333333333333332No Hit
CACACCAGCTGTGCGATGCCCGCTTTTACAATTAAAGGAAAACTGTTGTGTTCAGTGTCACCTTGTCAGCAACAC18.333333333333332No Hit
ATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTA18.333333333333332No Hit
GAGCCGCCCCCGGGTCCGCAGCGACCCGACCAGTCGGCCGCCGCCGCTGGCCCCGGGGATCCGAAGAGGAAGCTG18.333333333333332No Hit
TCTCCAAGCACACCAAACCAAACCACACCACATAAGCACACATAACATTAAAAAAAAATATTATACACATCTCCG18.333333333333332No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG18.333333333333332No Hit
CATCCGCAGCGTCATCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)