FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAGACAC213.333333333333334No Hit
TCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG213.333333333333334Illumina PCR Primer Index 6 (96% over 27bp)
CTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG16.666666666666667RNA PCR Primer, Index 7 (96% over 28bp)
GAAAACACACAAGCCAAAAAACCCAAGACTAACTCAGACTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.666666666666667No Hit
TTTCAGTTCCTTTCTAAGCTTTGTCAGTCAAGGGGCTCCACTGACTTCCTAGGCCCTGTAATTTAACCAGTCTTT16.666666666666667No Hit
TGATGAGATTGTCAACATTGCTCGACAGATGCGGCACCGATCCTTAGCCAGAGAACTCTCTGGAACCATTAAAGA16.666666666666667No Hit
AGGATATTATCTCATATGGCGGAGTAGAACGTCTTTAGCGGCCTGTCATATTAACAACCGTTGCGACGGGTCCGA16.666666666666667No Hit
CCCTGGGCCACTCTGCTGGGTGCTTAGTGAGCAAAGTTACAACCTTCTGGGCTGAGGTGACTCCAGCATTGAACG16.666666666666667No Hit
GTGAGTCAGCCTCTGCGCTCACTCTCCAGACTCTTCTAAACAAATCTCAAGGATCTGTTGGGAAGGGAAAGGCCA16.666666666666667No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC16.666666666666667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCATGCACTTGTAAACACGAAGACTACAAGGAACGATC16.666666666666667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG16.666666666666667Illumina PCR Primer Index 1 (96% over 28bp)
TTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCTGTCTCTTATACACATCTCCGAGCC16.666666666666667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)