FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGTCGATGAGGAGGCAGCGCTTGATGGTGTTCAGGTTCACCTTGTGCACGTTGATGGCTGTCTCTTATAC16.666666666666667No Hit
CTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG16.666666666666667RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACATCATCTCATCTCGTAAGCCGTCTTCTGCTTGAAAAAAAACAAGGGGGGGGGGGGGG16.666666666666667Illumina PCR Primer Index 7 (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGA16.666666666666667No Hit
AATATCAAAAAAAAAAACAACGATGAGCAGACAAACCACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.666666666666667No Hit
GTGTGGGATCCTAGAGAGTTTGCTGTTATGGGAACAGCAGGATGACTGGTTGCATGAGGGGTTTCCTGGGGCACT16.666666666666667No Hit
GCCCACCCCTTCTTCTTCACCATACCCCAGAATCTTCCATGCTCCGTCACACTGCAGCCAGGCCCAGAGGATACA16.666666666666667No Hit
CTCCGAGCCCACGAGACCCACGTATAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGGGCGGGGGGGGGCG16.666666666666667Illumina Single End Adapter 1 (95% over 22bp)
GCCTAAGCCTCCCGAGTAGCTGGGATTACAGGCAACTGCCACTTAAACTGGAGTTGTTTTTTTTTTCTTTGAGAC16.666666666666667No Hit
TCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.666666666666667RNA PCR Primer, Index 47 (96% over 30bp)
GTTGTTCTTTGCTTTATATACATAAGCGCATCTCTTGCCCAAATAGAATTCTGTTTCATCTCGGGCGTAAACACC16.666666666666667No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCCATGTAATATACGAAGAAACAGTCACAAAAAAACAAA16.666666666666667No Hit
CTCCGAGCCCACGAGACGTGAATTCATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGAGGTGGGGCGGGGG16.666666666666667RNA PCR Primer, Index 45 (96% over 28bp)
GAGTACATGGGCCCTAAGGTGAGGCCGAAAGGCGTAATCGATGGGAACCAGGTGAATATTCCTGGGCCAGTTGGA16.666666666666667No Hit
TTATTAAATTTTTATTCCTAAACTAAAAAGAAAAAAATTTAAAATATAAAAAAAAACCCCACCACCAGAACCAGA16.666666666666667No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)