FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGACAGGGTCACGTCCTTCTTGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT211.76470588235294No Hit
TCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.88235294117647Illumina Paired End PCR Primer 2 (96% over 28bp)
CCTGACCCCCGACCTCTGGAAGGAGACTGTATTCACCAAGTCTCCCTATCAGGAGTTCACTGACCACCTCGTCAA15.88235294117647No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGC15.88235294117647No Hit
GTAAATAAGAGAAATTAACATCAGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAACCAAATAAAAATAA15.88235294117647No Hit
TCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.88235294117647TruSeq Adapter, Index 4 (96% over 28bp)
CTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCATCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG15.88235294117647Illumina PCR Primer Index 1 (95% over 22bp)
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGATCAATCCTCA15.88235294117647No Hit
CACCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGCGGGGGGGGGGGG15.88235294117647RNA PCR Primer, Index 32 (96% over 33bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCCTAAGCCCACTTCAGCATGAAAAAAAAAACGGGG15.88235294117647No Hit
GTTCCTAAGGGTGCAACTTACGGCAAGCCTGTCCATCATGGTGTTAACCAGCTAAAGTTTGCTCGAAGCCTTCAG15.88235294117647No Hit
CTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG15.88235294117647Illumina PCR Primer Index 1 (96% over 28bp)
CAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGGTGGGGATGAGAGAAAGACATCAAAGTTTGAGAAG15.88235294117647No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATAGGCAAAAAAAACATGAAAATTAAAAAAAAC15.88235294117647No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGA15.88235294117647No Hit
GTATAAGAGACAGGATGTTGAAATGTCAGGGTCACGTCTTCTTGATCTGTCTCTTATACACATCTCCGAGCTGTC15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)