FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACAGGTGTGAGCCACCACACCCAGCCACTGTCTTCCTTTTAAACTAACACAGCTGAAGGGTGATAAATGAGGG16.25No Hit
ATTCCAGAGAGCTGTTGCGCAGCCATTGGTACCTGTCTCTTATACACATCTCCGAGCCCACAGACATCATCTCAT16.25No Hit
TCTTTGCGGGAGCCATTCTGCAGCGTCCAAGTTGGAAAGCCCCATGTACTCTGCGTTCTGTCTCTTATACACATC16.25No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT16.25No Hit
GACTACAGGCACCTGCTACCATGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCA16.25No Hit
GGTATCAACGCAGAGTACATGGGTGTCACTTTTAAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC16.25No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC16.25RNA PCR Primer, Index 47 (96% over 26bp)
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGCACACGCTTCTGTCCTGAGGTTCAAGCGAGGAGAGC16.25No Hit
CCCTGATTCCCCGTCACCCGTGGTCACAATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTC16.25No Hit
CATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG16.25RNA PCR Primer, Index 47 (96% over 30bp)
CTCCGAGCCCACGCGCCCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAATAAAAAGGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 37 (96% over 28bp)
GATGAAACCCAGACACATAGCAATTCAGGAAATTTGACTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAA16.25No Hit
TCTCCGAGCCCACGAGACCATCGACCATCGGGTATTGCGGCTTGTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25No Hit
GTCTAGGGATGCAGGATCCCCGCCCTGGGGTCTGGTCCTCGCCCATCCTGCAGGGATTGCCCACCGTCTTCCAGA16.25No Hit
CTTATACACATCTCCGAGCCCACGGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGATAAAAAAAATGGGGG16.25Illumina PCR Primer Index 1 (96% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGTACTCTGCGTTGATACCTGTCTCTTA16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)