FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAACCATCGACCA16.25No Hit
AATAAAAATATTAAAAAAATTTATAATAAAAAAATATAAAATAAAACTAAACTTATAAAAAAATAAAAATCAGAC16.25No Hit
CCATATACACAAAAAACAATCAATAAAACACATCACCGAACAAAAAAGAACCTCCGAAATCTCTAATGACTTCTG16.25No Hit
TCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25TruSeq Adapter, Index 7 (96% over 28bp)
CAGCTTGGCATAGTAGAAGAAACAAACCCAAAATGAAAACACCCGGATTTTAAGCGCGGATCCTGCGAGGCCGGT16.25No Hit
CTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25TruSeq Adapter, Index 1 (96% over 28bp)
GAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGC16.25No Hit
CGTGATAGGCGCCACACATGAGGGTCGACGATGACATCCGACACCGCCGGCCCGGATTCGCTGAAGTGAAAGAAC16.25No Hit
TCCGAGCCCACGAGACGTGCATACCGCGCGGATGCCGTCTTCTGCATGATAAAAAAAATGGGGGGGGGGGGGGGG16.25No Hit
CATCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG16.25RNA PCR Primer, Index 41 (96% over 29bp)
GGTGATGAGCTTGGGGTCGGTCATAAGCGCGGTGGCGTCGTCGCTGGGAGCTGGCAGGGCCTCCCGCAGGAAGGC16.25No Hit
GTATCAACGCAGAGTACATGGGAAAAAACTAGCAAAAAATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA16.25No Hit
ACAAAAGAGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAACGAATAAAAAGTGAGAAAAA16.25No Hit
GAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGGGGG16.25RNA PCR Primer, Index 36 (96% over 29bp)
CTCCGAGCCCACGAGACCAATCCTCATGTCGTATGCCGTCTTGTGCTTGAAAAAAAAATGGGGGGGGGGGGGGGG16.25Illumina DpnII expression Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)