FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGCCCACGAGACAACCAACGAGGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGG14.166666666666666Illumina PCR Primer Index 1 (95% over 22bp)
GTCCTAGTCTCCCTCCTCTTCGTCATGCTGCGCTACATGTACCGGCACAAGGGCACGTACCACACCAATGAGGCC14.166666666666666No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCAGAGCCCACGAGACCAATCCACATCTCGTATGCCGTC14.166666666666666No Hit
TCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG14.166666666666666TruSeq Adapter, Index 1 (96% over 28bp)
GAGCTGAGGTGAAGATCCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGATG14.166666666666666No Hit
CTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACTGGGGGGGGGGGGGGG14.166666666666666Illumina PCR Primer Index 10 (96% over 27bp)
TCTCTTTGTAATGCATGCTTGATTGCTGTCATCTTTTCTCAGCGGGACAAACGGTGAAAGGAAGGTCAGAAGGAG14.166666666666666No Hit
TCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG14.166666666666666Illumina PCR Primer Index 2 (96% over 28bp)
TCTCCGAGCCGACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG14.166666666666666RNA PCR Primer, Index 16 (96% over 29bp)
GCATCACTCAGCACATTGGTGCCTTTCTTGTCTCTCTGCCTTCTGGGGAAAAGATAACTTTTCTTGATACTCCAG14.166666666666666No Hit
CTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG14.166666666666666RNA PCR Primer, Index 1 (96% over 28bp)
CCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGG14.166666666666666No Hit
CATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG14.166666666666666RNA PCR Primer, Index 45 (96% over 30bp)
TCGCCTCCCGAAGTGCTGGGATTACAGATGTAAGTCACTACCCCAGCCAGAAACTAACATTCTCAAAAGCTACTA14.166666666666666No Hit
GTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTTCGGTCCAGTGCTTCCATCAGTCCAAACATCAAGTGTAAGT14.166666666666666No Hit
CTCCGAGCCCACGAGACCATCGACCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG14.166666666666666RNA PCR Primer, Index 14 (96% over 26bp)
GCAAGACACCCAAGTGCTGGGCTTGGATCCTGGAGACAAGGCAGAAGTAGAGGTTCTGTGAGCTTCTTTCTTTAC14.166666666666666No Hit
CGCTCATAGAGGCGAACCTCACACATCAAAGGCTGTGACACCCAGTGAATAAAGGCCTTTGGCTTCTCTCCAGCA14.166666666666666No Hit
CTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG14.166666666666666TruSeq Adapter, Index 25 (96% over 28bp)
TCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGGGGGG14.166666666666666TruSeq Adapter, Index 7 (96% over 28bp)
CTGAGCTTCCTGGGACAACAGAAGGCTGCATTTGTTGGCTGAGTTCACACAGAAGCAGGACTGCTCGGCCAGAAG14.166666666666666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14.166666666666666No Hit
GTGTAGGGCCGCGCGCCGTGGCCGCGTCACACGCCTCAGGCCGGTGCGCAAGTCGGCTCCCACGATTAGCCAGAT14.166666666666666No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC14.166666666666666No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)