FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCTCTGCTTCTGTAAGAATCCTGAATTTAGGATTTTCAACTGTCACTACTCCAACTTCTATTTCTGAA16.25No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGATTCAACGGGTTACCC16.25No Hit
TCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.25TruSeq Adapter, Index 1 (96% over 28bp)
CCCCCATCTCAGAACAGAAGGGTGGGAAGCCAGAGCCGCCTGCCATGCCCCAGCCAGTCCCCACAGCATAACAGC16.25No Hit
GTATCAACGCAGAGTACATGGGGTGGAAAGCGGGCCGCCCGGCATGATCGACGGGCCCTTGCGCGCGCCTTCCTC16.25No Hit
AGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16.25Illumina PCR Primer Index 5 (96% over 27bp)
AGCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC16.25No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACTTCTAAGGGCATCACAGAC16.25No Hit
GGTATCAACGCAGAGTACATGGGGATCTTCACATCAGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.25No Hit
CACAGGTGCTTCACAGTGTCTATGTCATCGTGGCAGAGGAGGCGCAGGCTGACCTCGCTGAGCGCGCTGGATGGC16.25No Hit
CCGTAACTCCGGCTCGGGCTCGAGACATTCTGACTTTACTTTTTCCAGATCAATGGCGGGACCGCTGCCAAGCCG16.25No Hit
GGCAAGCGCGGCTTGGAGGGCGGACGGGGCGCGGCGATACAGCTGCCCGCTCGCATCTTTGGCCCTGCGCTCAGC16.25No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATACTAGTGGCTAGCAATATATGGAAAAAACAAGAAC16.25No Hit
TAAAAATAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAACAACCAAACAAAAAAATAA16.25No Hit
ATCAACGCAGAGTACATGGGGGATCTTCACCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGT16.25No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)