FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.666666666666667TruSeq Adapter, Index 7 (96% over 28bp)
AATCAAATGTGAATAATAGACAACAACAGCGAAAAAAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.666666666666667No Hit
GAGGTTGGTTGATGAGGTTTAAGGAAAGAAGCCATCCTCATATCATACCCATGTACTCTGCGTTGATACCTGTCT16.666666666666667No Hit
TTTTAACACTGCTGTGAATGTGCTGGGGTCACCAAGGGGGCCCCTTAGTGGTCAGATGCACTTTGTCACAATGTA16.666666666666667No Hit
CATGTAAAGTTAGCGCCGGTGTTTGATCTGTTACAGGTAAAAATTCTTTATATTGATGGATAAGTCCTTTCCACA16.666666666666667No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGG16.666666666666667RNA PCR Primer, Index 29 (96% over 30bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGGCTGGATACCGCAGCTAGGAATCTGTCTC16.666666666666667No Hit
AAGCAGTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAAATGAA16.666666666666667No Hit
AGGCGAGCCACCGCCCGTCCCCGCCCCTTTCCACTCGGCCCCCTCGATGCTCTTAGCTGAGTGTACCGCCTTTAT16.666666666666667No Hit
GAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATCGGGGGGGGGGGGGGGGGGG16.666666666666667RNA PCR Primer, Index 36 (96% over 30bp)
TCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.666666666666667TruSeq Adapter, Index 4 (96% over 28bp)
GGGTTCGCCCGCGCCAGGTGCGCCACGGACGAGAACATCTTTTCCCTAAGATGGCGATCACACAACCACTCCCTT16.666666666666667No Hit
CATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG16.666666666666667TruSeq Adapter, Index 6 (96% over 28bp)
TCATCAAGGTGTTCAACGACATGAAGGTGCGTAAGTCTTCAACGCCAGAGGAGGTGAAGAAGCGCAAGAAGGCGG16.666666666666667No Hit
TCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG16.666666666666667Illumina PCR Primer Index 7 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)