FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT11.7857142857142856No Hit
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCC11.7857142857142856No Hit
CATGGTGTGGGCCCGGCCCTCCGTGCTGTTCCTGCCAATGGCATGGCTGATGGTTACCCGGCTCTCCCTGTCTCT11.7857142857142856No Hit
ACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGTACTCTGCGTTCTGTCTCTTATACACATCTC11.7857142857142856No Hit
GAGAAAAGAGTGTACAAACTTGATGGAATCACAACAGTCAATATAATTTAAGGGACAATAAAGTCAATAAGGTTG11.7857142857142856No Hit
TTGTTCGTCAGGTCGAACTCGAACATGCGCATGAGGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG11.7857142857142856No Hit
ATATTTAAGCACGATGAACACTTTTCGTGCTGAACAGGCATATTTTTAGGATACATTGTGTGCCTAACATTGTGC11.7857142857142856No Hit
ATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAAGTC11.7857142857142856No Hit
TGCGCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCTGTCTCTTAT11.7857142857142856No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTTGATCACGTT11.7857142857142856No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC11.7857142857142856No Hit
TCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG11.7857142857142856Illumina PCR Primer Index 10 (96% over 27bp)
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGACTT11.7857142857142856No Hit
GTGGAATACACCACTTGGTTTTTATATTTTGCATGTGCTTCACTATCAAAGTTGTTCATGTATCTGCAGAGATCC11.7857142857142856No Hit
GAGTGGCCCAGAAGGAAGACAGGAAGCCCAGGGTAGTCTTTCTGCATGGAATCCACATGCTGCAACACATCCCTG11.7857142857142856No Hit
GAAGATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT11.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTCCTGACATCTCTCTGAACAACGAGAAGGTGCTGGTGGAGACCAGTTACCCCAGCCAAACCACTCGACTGC11.7857142857142856No Hit
GAGCTGCAGGAAAAGAGGAGGCGAGAGGCTATCACTGCAGCGACCTGCCTGACAGAAGCTTTGGTGGATCACCTC11.7857142857142856No Hit
GAGGAAGCCCGATAGCGGTTCTCGGCCTCCGAAACACAACTACAACACTGGGGAGCTTGCCCTGCAGTTAGCGTC11.7857142857142856No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11.7857142857142856No Hit
GAGCTGGGAGGCTTCACAGAGAAGGCTGTGGTTCCCCTGGGCCTCTACACTGGGCAGCTGGCCCTGAACTGGGCA11.7857142857142856No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATAT11.7857142857142856No Hit
GCCCACCGGTCTGCTTGCCCAACGTTATTAGTGCCCCTATTGCTCTACAAATTCGAACCTCAAACACAAAACCAG11.7857142857142856No Hit
CGCTGATCCGCGACTACATCCTGCTCGACGGGCGCGCGGTGGCCTTCCTGGTCGACACCGGCGGCGAGATCAAGC11.7857142857142856No Hit
CCTAAGGGCCTTTTCCCCTCCTGCCTAGAAAATAGGTTGTGTATTGGTTTTATTCAACCTGCTGTCTGCTTTAGG11.7857142857142856No Hit
CCGCAGGGCCGATCACGCTGGGGGCGCTGAGGCCGGCCATGGTCATGGAAGTGGGCACCCTGGACGCTGGAGGCC11.7857142857142856No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTG11.7857142857142856No Hit
CGGAGATGTGTATAAGAGACAGTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAA11.7857142857142856RNA PCR Primer, Index 37 (96% over 28bp)
GGTGATGTACTCCACAGTGACCGGGTCCTCCACAGTCAGCCGGTGGCTCTGGCACTCCTGTCTCTTATACACATC11.7857142857142856No Hit
GTTAGAGATCTGTCAGAGAAGGCATTTCCTAAGTGGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTGG11.7857142857142856No Hit
GATCTACACTGCCGTGATCGTTATATGACGAGACTGTGCGCTCATAGCCGACACTGTGCTCGATAAGCTGTCTCT11.7857142857142856No Hit
GACAGAGGCAGGAGAAATGCTTGAATCCCGGAGGCGGAGGTTGCGGTGAGTCGAGACTGTCTCTTATACACATCT11.7857142857142856No Hit
GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAG11.7857142857142856No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAG11.7857142857142856No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAG11.7857142857142856No Hit
GCCCTGGGTTCTGCCCGCACGGACCCCCATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGCTGTCTCTTAT11.7857142857142856No Hit
CTCCCGCCCTGCTCGTTCGCTCTCCAGCTTGGGATGGCCGGCTACCTGCGGGTCTGTCTCTTATACACATCTCCG11.7857142857142856No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11.7857142857142856No Hit
CATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG11.7857142857142856TruSeq Adapter, Index 1 (96% over 28bp)
TCTACGAGCACACGAGACACAATTCATTAACGAAATCCTACATCTCATTCAAAAATAAAAATCTCCGAGCCCACG11.7857142857142856No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA11.7857142857142856No Hit
TTTCAGGTTAGACCTCATCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGA11.7857142857142856No Hit
TCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGG11.7857142857142856RNA PCR Primer, Index 36 (96% over 30bp)
ATTCTGGGCTTCCACACAACTCCAGTGCTAACTCAACAGAGACTCTCCAACATGTGCCTTCTGACCATACAAATG11.7857142857142856No Hit
ACATGCCATGAGGGCCATGGTGTGCAAGCTGCATCTGAGACATCCCTGGATGGGGCATCCCACCCGGTGGGAATG11.7857142857142856No Hit
GCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGGCTTCTCTCCCGCGGACGTCTTCGTGCAGTG11.7857142857142856No Hit
GTTTGCAGACCCCGGCGCTCTGCAGCGGCACGTCCGCATTCACACAGGTGAGAAGCCATGCCAGTGTGTGATGTG11.7857142857142856No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCA11.7857142857142856No Hit
CATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG11.7857142857142856RNA PCR Primer, Index 32 (96% over 33bp)
CTCTTCTACGCCATGGCCTCCGGCGATGCGCGTCCGCTCGCCGCCGCGATGAAGGTCACGAAGCCCCGGCCCGCC11.7857142857142856No Hit
GCCCCAGTCCAGCGACACCTCGCCCTTGCCCATCTTGGCGCGGTTGTTGACGACGTTCTCAACCAGCGGGTGCAG11.7857142857142856No Hit
GATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCACTGTCTCTTATACACATCTCCGAGCCCA11.7857142857142856No Hit
CTCTCTCACCAGGCTGGAGTGCAGTGGTGCAATTTCGGACTCAGCCCACTTGCACCCAAGTGAATACACAGCTTT11.7857142857142856No Hit
CCTTTGTGGTCTGCCCCAAACCCCATTTAGAGAGCAGAGTAAATTTTCATACAACACTGTGTCAGTGAGACTGGG11.7857142857142856No Hit
GCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC11.7857142857142856No Hit
GTCCAGGGTGGGCATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT11.7857142857142856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)