FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCAGCCTGGCCAACATAGCGAGACCCCCCTCTCTGCTATCTTTTTCCGGTAATTAAACCTTCCTCTTGTGCC15.0No Hit
TCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0TruSeq Adapter, Index 7 (96% over 28bp)
CTCACCAACGCGGCGGGGACACCGATCTATGCGGGGTCGATCAACAGCGGCGGCTACAATTATAACGTAGCCAAC15.0No Hit
TCTCCGAGCCCACGAGACGATGCTCAATATCGTATGCCGTCTTCAGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0RNA PCR Primer, Index 32 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0Illumina PCR Primer Index 2 (96% over 28bp)
GACCAGGGAGGACATTGGCACGCTGTCTCCAGTCTCTTATACACATCTCCGAGCCAACGAGACGCAGCAACATCA15.0No Hit
GGGCAGGACCGGTTCTCAGGGACAGTGCACGCAGGTGCGCGTGGAATTCATGGACGACACGAGCCGATCCATCAT15.0No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGCTAAGTGCTAAAATGAATGCCAGCTGTCTCTAATACAC15.0No Hit
GTCCAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG15.0No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGG15.0No Hit
ATCCTGGCCAACATGGCGAAACCTTATCTCTAATAAATACAAAAATTAGCCGGGCGTGGTGGCATGCACCTGTAG15.0No Hit
CATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGG15.0TruSeq Adapter, Index 6 (96% over 28bp)
CGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAGC15.0No Hit
ATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGG15.0Illumina PCR Primer Index 2 (96% over 28bp)
CTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG15.0Illumina PCR Primer Index 6 (96% over 27bp)
CTCCGAGCCCACGAGACAAGCGTGTATCTGGGATGGCGGGTTCTGCTTTTAAAAAAAAAGGGGGGGGGGGGGGGG15.0No Hit
CTCCGAGCCCACGAGACCAATCCTCCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG15.0TruSeq Adapter, Index 23 (96% over 27bp)
TCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.0RNA PCR Primer, Index 32 (96% over 33bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAACACTAAAACAAGAAAAAAAAAAAAAAA15.0No Hit
GAAGAGGTGCTGACAGTGGGGGAGACAGGGAGAAAGAAAGAGGCTGAGAGAGAAAGAGAGACGGAGAGCTGAGAT15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)