FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG17.6923076923076925Illumina Paired End PCR Primer 2 (96% over 28bp)
GCCTTAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCATCCCTGTTTTAATCCATCTGACATATTTCTTCTG17.6923076923076925No Hit
CTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG17.6923076923076925RNA PCR Primer, Index 1 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAACACCATTCAATCTCGTATGC17.6923076923076925No Hit
TCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGGGGGG17.6923076923076925Illumina Paired End PCR Primer 2 (96% over 28bp)
TCTCCGAGCCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGG17.6923076923076925RNA PCR Primer, Index 32 (96% over 33bp)
CCTCGGGAGCCAGGGCTGTGCACCTGGCAGACCCTGCGGTCCCAGATAGCCTGAATCCAGCCCCTGTCTCTTATA17.6923076923076925No Hit
CATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG17.6923076923076925TruSeq Adapter, Index 4 (96% over 28bp)
ACACCTAACCAACAATACCTGAATAAAAAACAAATCACCTATACAACTAGAAAAAAAATAATGTCGTATGCCGTC17.6923076923076925No Hit
CGCCTTCGTACCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGCACCATTCAA17.6923076923076925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17.6923076923076925No Hit
TCTCCGAGCCCACTAGACAAGCGTGTATCTCGTTTGCCGTCTTCTGCTTGAAAAAAATATCGGGGGGGGGGGGGG17.6923076923076925TruSeq Adapter, Index 2 (96% over 27bp)
ACGCAGAGTACATGGGTGATCGTCGCCACCACCGATGGGGTCGGCACCAAGCTCAAGATCTGTCTCTTATACACA17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)